DR3 protein

DR3-muIg-Biotin

Cat. Num. AAA666875

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Reactivity: Human
• Applications: ELISA
• Synonyms: DR3; DR3-muIg-Biotin; Human DR3-muIg/Biotin; DR3 protein

• Host: CHO cells
• Reactivity: Human
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 0.4 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for DR3 protein: ELISA (EIA)
• Molecular Structure: A soluble dimeric fusion protein consisting of the extracellular (150aa) domain of human DR3 fused to murine IgG2a Fc (233aa). Predicted non glycosylated monomeric molecular weight is 42.9 kd. N-teminal sequence: (27) GTRSP
• Transfectant Cell Line: CHO
• Performance: Identity of DR3-muIg was confirmed by n-terminal sequencing: (27)GTRSP. DR3-muIg/Biotin was detectable at 5 ng/ml in EIA using anti-DR3 antibody coated plate to capture, and Streptavidin/HRP as a detection reagent.
• Conjugation: Biotin
• Production: Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 6 months after shipping date when stored as recommended.

Testing Data

Related Product Information for DR3 protein

Information: Human DR3 (TRAMP, LARD) was redesignated TNF receptor superfamily member 25. TL1A is a ligand which can induce apoptosis through a cytoplasmic death domain similar to the CD178-CD95 (FasL-Fas) interaction. Alternatively, engagement of DR3 on T cells can synergize with other activating signals to enhance IFN? production. Recombinant DR3-muIg binds recombinant TL1A in EIA.

References

(1) A Kaptein, et al. (2000) FEBS Lett 485:135. (2) T Migone, et al. (2002) Immunity 16:479. (3) S Targin, et al. (2004) 172: 7002.

NCBI and Uniprot Product Information

• NCBI GI #: 37181738
• NCBI GeneID: 8718
• Molecular Weight: 29,111 Da
• NCBI Official Full Name: DR3
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25; protein WSL-1; death receptor beta; apoptosis inducing receptor; apoptosis-inducing receptor AIR; apoptosis-mediating receptor DR3; apoptosis-mediating receptor TRAMP; death domain receptor 3 soluble form; lymphocyte-associated receptor of death; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytosol

Molecular Function: tumor necrosis factor receptor activity; receptor activity

Biological Process: regulation of apoptosis; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction