Typical Testing Data/Standard Curve (for reference only)
Mouse Enoyl-CoA hydratase, mitochondrial ELISA Kit | Echs1 elisa kit
Mouse Enoyl-CoA hydratase, mitochondrial ELISA Kit
Gene Names
Echs1; C80529
Reactivity
Mouse
Synonyms
Enoyl-CoA hydratase; mitochondrial; Mouse Enoyl-CoA hydratase; mitochondrial ELISA Kit; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH; Echs1; 4.2.1.17; Echs1 elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouse Enoyl-CoA hydratase, mitochondrial. No significant cross-reactivity or interference was observed.
Sequence Length
290
Assay Type
Sandwich
Detection Range
78-5000 pg/mL
Sensitivity
39 pg/mL
Intra-Assay CV
<=4.4%
Inter-Assay CV
<=6.6%
Recovery
102%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Echs1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Enoyl-CoA hydratase, mitochondrial concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enoyl-CoA hydratase, mitochondrial. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Enoyl-CoA hydratase, mitochondrial and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Enoyl-CoA hydratase, mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Enoyl-CoA hydratase, mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enoyl-CoA hydratase, mitochondrial. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Enoyl-CoA hydratase, mitochondrial and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Enoyl-CoA hydratase, mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Enoyl-CoA hydratase, mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,474 Da
NCBI Official Full Name
enoyl-CoA hydratase, mitochondrial
NCBI Official Synonym Full Names
enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
NCBI Official Symbol
Echs1
NCBI Official Synonym Symbols
C80529
NCBI Protein Information
enoyl-CoA hydratase, mitochondrial
UniProt Protein Name
Enoyl-CoA hydratase, mitochondrial
Protein Family
UniProt Gene Name
Echs1
UniProt Synonym Gene Names
SCEH
UniProt Entry Name
ECHM_MOUSE
Uniprot Description
ECHS1: Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Belongs to the enoyl-CoA hydratase/isomerase family.
Protein type: EC 4.2.1.17; Other Amino Acids Metabolism - beta-alanine; Lyase; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - fatty acid; Lipid Metabolism - fatty acid elongation in mitochondria; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - propanoate; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation
Cellular Component: mitochondrion
Molecular Function: catalytic activity; enoyl-CoA hydratase activity; lyase activity
Biological Process: fatty acid metabolic process; lipid metabolic process; metabolic process
Research Articles on Echs1
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Product Notes
The Mouse Echs1 echs1 (Catalog #AAA2886330) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886330 ELISA Kit recognizes Mouse Echs1. It is sometimes possible for the material contained within the vial of "Enoyl-CoA hydratase, mitochondrial, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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