Mouse Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit | EAAT2 elisa kit

Mouse Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit

Cat. Num. AAA2019055

• Gene Names: SLC1A2; HBGT; EAAT2; GLT-1
• Reactivity: Mouse
• Synonyms: Excitatory Amino Acid Transporter 2 (EAAT2); Mouse Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit; SLC1A2; EAAT2; GLT-1; Solute Carrier Family 1 Member 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; EAAT2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Excitatory Amino Acid Transporter 2 (EAAT2).; No significant cross-reactivity or interference between Excitatory Amino Acid Transporter 2 (EAAT2) and analogues was observed.
• Sequence Length: 565

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EAAT2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 2 (EAAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 2 (EAAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EAAT2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 307746865
• NCBI GeneID: 6506
• NCBI Accession #: NP_001182657.1
• NCBI GenBank Nucleotide #: NM_001195728.2
• UniProt Accession #: P43004; Q14417; Q541G6; B4DQE9; U3KQQ4
• Molecular Weight: 61,008 Da
• NCBI Official Full Name: excitatory amino acid transporter 2 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 2
• NCBI Official Symbol: SLC1A2
• NCBI Official Synonym Symbols: HBGT; EAAT2; GLT-1
• NCBI Protein Information: excitatory amino acid transporter 2
• UniProt Protein Name: Excitatory amino acid transporter 2
• UniProt Gene Name: SLC1A2
• UniProt Synonym Gene Names: EAAT2; GLT1
• UniProt Entry Name: EAA2_HUMAN

NCBI Description

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]

Uniprot Description

GLT1: an integral membrane protein belonging to the sodium:dicarboxylate (SDF) symporter family. Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by co-transporting sodium.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p13-p12

Cellular Component: membrane; integral to membrane; plasma membrane; axolemma

Molecular Function: protein binding; sodium:dicarboxylate symporter activity; glutamate:sodium symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; multicellular organism growth; multicellular organismal aging; response to amino acid stimulus; visual behavior; positive regulation of glucose import; synaptic transmission; L-glutamate import; telencephalon development; adult behavior; response to wounding; cellular response to extracellular stimulus; ion transport; transmembrane transport