E2F Transcription Factor 2 Recombinant Protein | E2F2 recombinant protein

Recombinant E2F Transcription Factor 2 (E2F2)

Cat. Num. AAA2033367

• Gene Names: E2F2; E2F-2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: E2F Transcription Factor 2; Recombinant E2F Transcription Factor 2 (E2F2); E2F2 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: RY DTSLGLLTKK FIYLLSESEDGVLDLNWAAE VLDVQKRRIY DITNVLEGIQ LIRKKAKNNI QWVGRGMFEDPTRPGKQQQL GQELKELMNT EQALDQLIQS CSLSFKHLTE DKANKRLAYVTYQDIRAVGN FKEQTVIAVK APPQTRLEVP DRTEDNLQIY LKSTQGPIEVYLCPEEVQEP DSPSEEPLPS TSTLCPSPDS AQPSSSTDPS IMEPTASSVPAPAPTPQQAP PPPSLVPLEA TDSLLELPHP LLQQTEDQFL SPTLACSSPLISFSPSLDQD DYLWGLEAGE GISDLFDSY
• Sequence Length: 437

• Applicable Applications for E2F2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 63.4kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Arg129~Tyr429
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31657162
• NCBI GeneID: 1870
• Molecular Weight: 47,506 Da
• NCBI Official Full Name: E2F transcription factor 2
• NCBI Official Synonym Full Names: E2F transcription factor 2
• NCBI Official Symbol: E2F2
• NCBI Official Synonym Symbols: E2F-2
• NCBI Protein Information: transcription factor E2F2
• UniProt Protein Name: Transcription factor E2F2
• Protein Family: Transcription factor
• UniProt Gene Name: E2F2
• UniProt Synonym Gene Names: E2F-2
• UniProt Entry Name: E2F2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F2: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: DNA binding; protein binding; transcription factor activity; transcription factor binding

Biological Process: mitotic cell cycle; regulation of cell cycle; regulation of transcription, DNA-dependent; transcription initiation from RNA polymerase II promoter