Chikungunya Mutant E1 Recombinant Protein | CHIKV recombinant protein
Recombinant Chikungunya Mutant (A226V) E1
Purity
Protein is >=95% pure as determined by 12.5% SDS-PAGE
Synonyms
Chikungunya Mutant E1; Recombinant Chikungunya Mutant (A226V) E1; CHIKV Mutant; Chikungunya Mutant (A226V) E1 Recombinant; CHIKV recombinant protein
Host
Insect Cells
Purity/Purification
Protein is >=95% pure as determined by 12.5% SDS-PAGE
Form/Format
CHIKV Mutant protein solution in 1xD-PBS, pH7.4, 0.1% Thimerosal, 5mM EDTA, 1 ug/ml of Leupeptin, Aprotinin and Pepstatin A.
Sterile filtered colorless solution.
Sterile filtered colorless solution.
Sequence
YEHVTVIPNTVGVPYKTLVNRPGYSPMVLEMELLSVTLEPTLSLDYITCEYKTVIPSPYVKCCGTAECKDKNLPDYSCKVFTGVYPFMWGGAYCFCDAENTQLSEAHVEKSESCKTEFASAYRAHTASASAKLRVLYQGNNITVTAYANGDHAVTVKDAKFIVGPMSSAWTPFDNKIVVYKGDVYNMDYPPFGAGRPGQFGDIQSRTPESKDVYANTQLVLQRPAVGTVHVPYSQAPSGFKYWLKERGASLQHTAPFGCQIATNPVRAVNCAVGNMPISIDIPEAAFTRVVDAPSLTDMSCEVPACTHSSDFGGVAIIKYAASKKGKCAVHSMTNAVTIREAEIEVEGNSQLQISFSTALASAEFRVQVCSTQVHCAAECHPPKDHIVNYPASHTTLGQDISATAMSWVQKITGG
Sequence Length
923
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Related Product Information for CHIKV recombinant protein
Description: Recombinant Chikungunya Mutant (A226V) E1 produced in Insect Cells is a polypeptide chain containing amino acids 1-415, however at position 226 the Alanine of the wild-type CHIKV E1 gene was mutated to Valine. The molecular weight of the CHIKV Mutant is approximately 50kDa. The E1 protein is C-terminal part of E2-6K-E1 protein region. CHIKV Mutant is purified by proprietary chromatographic technique.
Introduction: Chikungunya virus (CHIKV) is an arthropod-born virus which is a member of the Alphavirus genus belonging to the Togaviridae family. CHIKV nucleocapsid is comprised of a single-stranded plussense RNA genome of approximately 11.8 kb. The CHIKV virion envelope consists of a lipid bilayer derived from the plasma membrane from the host cell, multiple copies of 2 major virus encoded glycoproteins E1 and E2, and a small 6K peptide. Proteins E1 and E2 both have a molecular weight of roughly 50kDa and form a heterodimer anchored in the membrane. Chikungunya virus infection causes an illness with symptoms similar to those of the dengue fever with an acute febrile phase lasting only 2-5 days, followed by a prolonged arthralgic disease affecting the joint extremities. Recent Chikungunya virus outbreaks presented a prospect for genetic analysis of patients with the illness, revealing a point mutation at the amino acid 226 (Ala mutated to Val) of the E1 gene. This point mutation was confirmed to be responsible for an improved capacity of CHIKV strains to infect and replicate in the Aedes albopictus, enabling virus transmission to a naive human population.
Introduction: Chikungunya virus (CHIKV) is an arthropod-born virus which is a member of the Alphavirus genus belonging to the Togaviridae family. CHIKV nucleocapsid is comprised of a single-stranded plussense RNA genome of approximately 11.8 kb. The CHIKV virion envelope consists of a lipid bilayer derived from the plasma membrane from the host cell, multiple copies of 2 major virus encoded glycoproteins E1 and E2, and a small 6K peptide. Proteins E1 and E2 both have a molecular weight of roughly 50kDa and form a heterodimer anchored in the membrane. Chikungunya virus infection causes an illness with symptoms similar to those of the dengue fever with an acute febrile phase lasting only 2-5 days, followed by a prolonged arthralgic disease affecting the joint extremities. Recent Chikungunya virus outbreaks presented a prospect for genetic analysis of patients with the illness, revealing a point mutation at the amino acid 226 (Ala mutated to Val) of the E1 gene. This point mutation was confirmed to be responsible for an improved capacity of CHIKV strains to infect and replicate in the Aedes albopictus, enabling virus transmission to a naive human population.
Product Categories/Family for CHIKV recombinant protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
101,424 Da
UniProt Protein Name
Structural polyprotein, E2-6K-E1 region
UniProt Gene Name
E2-6K-E1
UniProt Entry Name
G0LWX6_CHIKV
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Product Notes
The CHIKV e2-6k-e1 (Catalog #AAA144891) is a Recombinant Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: YEHVTVIPNT VGVPYKTLVN RPGYSPMVLE MELLSVTLEP TLSLDYITCE YKTVIPSPYV KCCGTAECKD KNLPDYSCKV FTGVYPFMWG GAYCFCDAEN TQLSEAHVEK SESCKTEFAS AYRAHTASAS AKLRVLYQGN NITVTAYANG DHAVTVKDAK FIVGPMSSAW TPFDNKIVVY KGDVYNMDYP PFGAGRPGQF GDIQSRTPES KDVYANTQLV LQRPAVGTVH VPYSQAPSGF KYWLKERGAS LQHTAPFGCQ IATNPVRAVN CAVGNMPISI DIPEAAFTRV VDAPSLTDMS CEVPACTHSS DFGGVAIIKY AASKKGKCAV HSMTNAVTIR EAEIEVEGNS QLQISFSTAL ASAEFRVQVC STQVHCAAEC HPPKDHIVNY PASHTTLGQD ISATAMSWVQ KITGG. It is sometimes possible for the material contained within the vial of "Chikungunya Mutant E1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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