Thioredoxin Reductase 1 Active Protein | TXNRD1 active protein

Recombinant Human Thioredoxin Reductase 1

Cat. Num. AAA144065

• Gene Names: TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Thioredoxin Reductase 1; Recombinant Human Thioredoxin Reductase 1; TXNRD1 Human; Thioredoxin Reductase 1 Human Recombinant; Thioredoxin reductase 1 cytoplasmic; TR; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein; KM-102-derived reductase-like factor; Thioredoxin reductase TR1; TXNRD1; GRIM12; KDRF; TR1; TXNR; TRXR1; MGC9145; TXNRD1 active protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 0.5mg/ml solution containing 1xPBS pH-7.4 & 10% glycerol
• Sequence: MGSSHHHHHH SSGLVPRGSHMYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQALQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLGIPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQIEAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQAGRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGC
• Sequence Length: 649

• Physical Appearance: Sterile filtered colorless solution.
• Biological Activity: Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5'-Dithiobis(2-nitrobenzoic acid)(DTNB)and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
• Preparation and Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for TXNRD1 active protein

TXNRD1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 508 amino acids (161-647 a.a.) and having a molecular mass of 55.7 kDa. TXNRD1 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

Product Categories/Family for TXNRD1 active protein

Enzymes; Reductase

NCBI and Uniprot Product Information

• NCBI GI #: 148277071
• NCBI GeneID: 7296
• NCBI Accession #: NP_001087240.1
• NCBI GenBank Nucleotide #: NM_001093771.2
• UniProt Accession #: Q16881; Q6FI31; Q6VB40; Q6VB41; Q6VB42; Q6VBP2; Q6VBP3; B7Z1F4; B7Z3Y8; B7Z904; E9PMY9; F5H780
• NCBI Official Full Name: thioredoxin reductase 1, cytoplasmic isoform 3
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: TXNRD1
• NCBI Official Synonym Symbols: TR; TR1; TXNR; TRXR1; GRIM-12
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; oxidoreductase; thioredoxin reductase GRIM-12; thioredoxin reductase TR1
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein
• UniProt Entry Name: TRXR1_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: Nucleotide Metabolism - pyrimidine; EC 1.8.1.9; Nuclear receptor co-regulator; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: protein binding; thioredoxin-disulfide reductase activity; electron carrier activity; FAD binding; protein disulfide oxidoreductase activity

Biological Process: response to reactive oxygen species; nucleobase, nucleoside and nucleotide metabolic process; cell redox homeostasis; nucleobase, nucleoside and nucleotide interconversion; cellular lipid metabolic process; signal transduction