Thymic Stromal Lymphopoietin Active Protein | TSLP active protein

Human Thymic Stromal Lymphopoietin (TSLP)

Cat. Num. AAA400081

• Purity: Greater than 97.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Synonyms: Thymic Stromal Lymphopoietin; Human Thymic Stromal Lymphopoietin (TSLP); TSLP active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ
• Sequence Length: 159

• Solubility: It is recommended to reconstitute the lyophilized TSLP in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.14 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: Less than 3%
• Endotoxin: Less than 0.5 EU (0.05 ng) per one microgram
• Formulation: Lyophilized from a concentrated solution (1mg/ml) contains 130mM NaCl, and 20mM sodium phosphate, pH 7.4.
• Biological Activity: The biologicsal activity was determined by the ability to induce proliferation of mouse BaF3 cells co-transfected with human Interleukin-7 receptor alpha & human TSLP Receptor, The EC50 was less than 0.3ng/ml.
• Preparation and Storage: Lyophilized TSLP although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TSLP should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please prevent freeze-thaw cycles.

Related Product Information for TSLP active protein

TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c(+) dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c(+) dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-alpha whereas down-regulating Interleukin-10 and IFN-gamma play a role in the initiation of allergic inflammation. Human recombinant TSLP is produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa. TSLP is purified by proprietary chromatographic techniques.

Product Categories/Family for TSLP active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 14719428
• NCBI GeneID: 85480
• NCBI Accession #: NP_149024.1
• NCBI GenBank Nucleotide #: NM_033035.4
• UniProt Accession #: Q969D9; Q8IW99; Q96AU7; G3XAM8
• Molecular Weight: 7,426 Da
• NCBI Official Full Name: thymic stromal lymphopoietin isoform 1
• NCBI Official Synonym Full Names: thymic stromal lymphopoietin
• NCBI Official Symbol: TSLP
• NCBI Protein Information: thymic stromal lymphopoietin
• UniProt Protein Name: Thymic stromal lymphopoietin
• Protein Family: Thymic stromal lymphopoietin
• UniProt Gene Name: TSLP
• UniProt Entry Name: TSLP_HUMAN

NCBI Description

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity