Uracil DNA Glycosylase Enzyme | UNG enzyme
Uracil DNA Glycosylase (UNG)
Gene Names
UNG; ATUNG; UNG; uracil dna glycosylase
Purity
Molecular Biology Grade
Synonyms
Uracil DNA Glycosylase; Uracil DNA Glycosylase (UNG); UNG enzyme
Host
E Coli strain K12
Purity/Purification
Molecular Biology Grade
Application Notes
Suitable for GMPD detection, Site-directed mutagenesis (2), as a Probe for protein-DNA interaction (3), Rapid, efficient cloning of PCR products (4) anddH2Oelimination of carry over contamination in PCR., Note:, The abasic sites formed in DNA by uracil-DNA glycosylase may be cleaved by heat under alkaline conditions. Any of the five MyBioSource buffers can be used instead of 10X uracil-DNA glycosylase reaction buffer. Uracil-DNA glycosylase should be inactivated by heating at 95 degree C for 10 minutes. Enzyme activity is partially restored at temperatures lower than 55 degree C. Put PCR products on ice after PCR and load directly on a gel.
Quality Control
Endodeoxyribonuclease Assay:
No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 10u of Uracil DNA Glycosylase with 1ug of pUC19 DNA in 50ul of reaction buffer (33mM Tris- acetate (pH7.9 at 37ºC), 10mM Mg-acetate, 66mM K-actetate) for 4 hours at 37 degree C.
No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 10u of Uracil DNA Glycosylase with 1ug of pUC19 DNA in 50ul of reaction buffer (33mM Tris- acetate (pH7.9 at 37ºC), 10mM Mg-acetate, 66mM K-actetate) for 4 hours at 37 degree C.
Storage Buffer
Supplied as a liquid in 30mM Tris-HCl, pH 7.5, 150mM sodium chloride, 1mM EDTA, 1mM DTT, 0.05% Tween 20, 50% glycerol.
Unit Definition
1unit of Uracil DNA Glycosylase catalyses the release of 1nanomole of uracil from uacil containing DNA template in 60 min at 37 degree .
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for UNG enzyme
Catalyzes the hydrolysis of N-glycosylic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. It shows no activity on RNA (1).
Product Categories/Family for UNG enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25.6kD monomer
NCBI Official Full Name
uracil dna glycosylase
NCBI Official Symbol
UNG
NCBI Official Synonym Symbols
ATUNG; UNG; uracil dna glycosylase
NCBI Protein Information
uracil dna glycosylase
UniProt Protein Name
At3g18630
Protein Family
UniProt Gene Name
UNG
UniProt Entry Name
Q9LIH6_ARATH
NCBI Description
Encodes a uracil-DNA glycosylase (UDG) involved in a base excision DNA repair pathway in mitochondria.
Uniprot Description
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. More active on U:G, U:T and U:C mispairs than on U:A pairs. Highly specific for uracil and no activity with 5-substituted uracil or cytosine derivatives. Required for initiation of base excision repair (BER) of uracil.
Research Articles on UNG
Similar Products
Product Notes
The UNG ung (Catalog #AAA653683) is an Enzyme produced from E Coli strain K12 and is intended for research purposes only. The product is available for immediate purchase. Suitable for GMPD detection, Site-directed mutagenesis (2), as a Probe for protein-DNA interaction (3), Rapid, efficient cloning of PCR products (4) anddH2Oelimination of carry over contamination in PCR., Note:, The abasic sites formed in DNA by uracil-DNA glycosylase may be cleaved by heat under alkaline conditions. Any of the five MyBioSource buffers can be used instead of 10X uracil-DNA glycosylase reaction buffer. Uracil-DNA glycosylase should be inactivated by heating at 95 degree C for 10 minutes. Enzyme activity is partially restored at temperatures lower than 55 degree C. Put PCR products on ice after PCR and load directly on a gel. Researchers should empirically determine the suitability of the UNG ung for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Uracil DNA Glycosylase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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