Human Caveolin 1 (CAV1) ELISA Kit | CAV1 elisa kit

Human Caveolin 1 (CAV1) ELISA Kit

Cat. Num. AAA455661

• Gene Names: CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• Reactivity: Human
• Synonyms: Caveolin 1 (CAV1); Human Caveolin 1 (CAV1) ELISA Kit; VIP21; Caveolae Protein 22kDa; CAV1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CAV1. No significant cross-reactivity or interference between CAV1 and analogues was observed.
• Sequence Length: 178

• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.234-15ng/mL
• Sensitivity: 0.07ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CAV1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CAV1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAV1 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CAV1 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAV1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CAV1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAV1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CAV1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15451856
• NCBI GeneID: 857
• NCBI Accession #: NP_001744.2
• NCBI GenBank Nucleotide #: NM_001753.4
• UniProt Accession #: Q03135; Q9UGP1; Q9UNG1; Q9UQH6
• Molecular Weight: 17,023 Da
• NCBI Official Full Name: caveolin-1 isoform alpha
• NCBI Official Synonym Full Names: caveolin 1
• NCBI Official Symbol: CAV1
• NCBI Official Synonym Symbols: CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• NCBI Protein Information: caveolin-1
• UniProt Protein Name: Caveolin-1
• Protein Family: Caveolin
• UniProt Gene Name: CAV1
• UniProt Synonym Gene Names: CAV

NCBI Description

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Uniprot Description

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed:25893292).