sRANKL Active Protein | sRANK active protein

Rat sRANKL

Cat. Num. AAA695952

• Gene Names: Tnfsf11; RANKL
• Reactivity: Species Reactivity: Rat; Cross Reactivity: Mouse
• Purity: Above 98% as determined by SDS-PAGE Analysis.
• Synonyms: sRANKL; Rat sRANKL; Recombinant Rat sRANK Ligand; sRANK active protein

• Host: E Coli
• Reactivity: Species Reactivity: Rat; Cross Reactivity: Mouse
• Purity/Purification: Above 98% as determined by SDS-PAGE Analysis.
• Sequence: PAMMEGSWL DVARRGKPE AQPFAHLTI NAADIPSGS HKVSLSSWY HDRGWAKIS NMTLSNGKL RVNQDGFYY LYANICFRH HETSGSVPA DYLQLMVYV VKTSIKIPS SHNLMKGGS TKNWSGNSE FHFYSINVG GFFKLRAGE EISVQVSNP SLLDPDQDA TYFGAFKVQ DID

• Biological Activity: Determined by its ability to induce NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.
• Cell Type: T Cells, Dendritic Cells
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for sRANK active protein

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant rat sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues).

Product Categories/Family for sRANK active protein

Adaptive Immunity; Apoptosis; Angiogenesis; Bone Biology

NCBI and Uniprot Product Information

• NCBI GI #: 21362971
• NCBI GeneID: 117516
• UniProt Accession #: Q9ESE2; Q91ZI9
• Molecular Weight: 35,370 Da
• NCBI Official Full Name: Tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: RANKL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11; ODF; OPGL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_RAT

NCBI Description

induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]

Uniprot Description

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.