stem cell factor Active Protein | rSCF active protein

Rat stem cell factor (rSCF)

Cat. Num. AAA400085

• Gene Names: KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7
• Purity: Over 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (c) Gel filtration chromatography under non denaturing conditions.
• Synonyms: stem cell factor; Rat stem cell factor (rSCF); rSCF active protein

• Host: E Coli
• Purity/Purification: Over 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (c) Gel filtration chromatography under non denaturing conditions.
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Glu-Ile-Cys.
• Sequence Length: 37

• Solubility: It is recommended to reconstitute the lyophilized rSCF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions conteining buffers and carrier proteins.
• Protein Content: Protein content:Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: This is a dimeric non-covalent protein. It has less than 3% monomers as determined by gel filtration chromatography under non denaturing conditions.
• Endotoxin: Less than 0.02 ng/ug (IEU/ug) of rSCF.
• Formulation: Lyophilized from a concentrated (1 mg/ml) solution in water containing 0.02% NaHCO3.
• Biological Activity: rSCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is less then 2 ng/ml, corresponding to a Specific Activity of approximetaly 100 IU/mg.
• Preparation and Storage: Lyophilized rSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution rSCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for rSCF active protein

Recombinant rat SCF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18407 Dalton. Bioactive rat SCF appears as a non covalent homodimer.

Product Categories/Family for rSCF active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1246100
• NCBI GeneID: 4254
• UniProt Accession #: P21583; Q16487; Q68DZ2; Q7M4N8; Q9UQK7; A0AV09; A8K2Q4; B7ZLM4
• Molecular Weight: 26,667 Da
• NCBI Official Full Name: stem cell factor, partial
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7
• NCBI Protein Information: kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor
• UniProt Protein Name: Kit ligand
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular space; cytoskeleton; cytoplasm; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; male gonad development; germ cell programmed cell death; signal transduction; positive regulation of MAP kinase activity; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mast cell apoptosis; positive regulation of melanocyte differentiation; innate immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7