Testing Data
()
Eco47I Restriction Enzyme
Eco47I (AvaII)
Purity
Molecular Biology Grade
Synonyms
Eco47I; Eco47I (AvaII); Eco47I restriction enzyme
Host
E Coli RFL47
Purity/Purification
Molecular Biology Grade
Sequence
5'-G^G A C C-3'
3'-C C T G^G-5'
3'-C C T G^G-5'
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
Supplied With
R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, pH 7.9 at 37ºC, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA
Note
Eco47I is blocked by overlapping dcm methylation. To avoid dcm methylation, use a dam, dcm strain such as GM2163.
Number of Recognition Sites in DNA
Lambda35
chi174,M13mp18/19 1
pBR3228
pUC57, pUC18/19, pTZ19R/U 2
chi174,M13mp18/19 1
pBR3228
pUC57, pUC18/19, pTZ19R/U 2
Storage Buffer
Supplied as a liquid in 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
()
Testing Data
()
Product Categories/Family for Eco47I restriction enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Eco47I restriction endonuclease
Similar Products
Product Notes
The Eco47I (Catalog #AAA638352) is a Restriction Enzyme produced from E Coli RFL47 and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: 5'-G^G A C C-3' 3' -C C T G^G-5'. It is sometimes possible for the material contained within the vial of "Eco47I, Restriction Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
