BpiI Restriction Enzyme

BpiI (BbvII)

Cat. Num. AAA638457

• Purity: Molecular Biology Grade
• Synonyms: BpiI; BpiI (BbvII); BpiI restriction enzyme

• Purity/Purification: Molecular Biology Grade
• Concentration: lOu/ul (varies by lot)

• Source: Bacillus pumillus Sw 4-3
• Digestion of Agarose-embedded DNA: A minimum of 5units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Methylation Effects: Dam, Dcm, EcoKl: never overlaps - no effect; CpG may overlap - no effect; EcoBl: may overlap-effect not determined
• Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BpiI.
• Blue/White Cloning Assay: The B/W assay was relpaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test.
• Labeled Oligonucleotide (LO) Assay: No detectable degradation of single-stranded or double-stranded labeled oligonucleotide occurred duringincubation with 10 units of Bpil for 4 hours.
• Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
• Number of Recognition Sites in DNA: Lambda: 24; PhiX174: 3; pBR322: 3; pUC57: 0; pUC18/19: 0; pTZ19R/U: 0; M13mp18/19: 0
• Stability During Prolonged Incubation: A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
• Diluent Buffer: 10mM Tris-HCl, pH 7.4 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Thermal Inactivation: Bpil is inactivated by incubation at 65 degree C for 20 minutes.
• Unit Definition: One unit is defined as the amount of BpiI required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of reaction buffer.
• Supplied With: MBS638383: Restriction Enzyme Buffer A, 10X, Dilute to 1X. 1X buffer compostion is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.; MBS638414: Restriction Enzyme Buffer C, 10X, Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 50mM sodium chloride, 0.1mg/ml BSA.
• Protocol for Digestion: Water nuclease free: 16u; MBS638414: 2ul; DNA(0.5-1ug/ul): 1 ul; MBS638457: 0.5-2UL; Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours. The digestation reaction may be either scaled up or down.
• Protocol for Digestion of PCR products Directly Amplification: PCR Reaction Mixture: 10ul (about 0.1-0.5ug of DNA); Water, nuclease free: 18ul; MBS638414: 2ul; MBS638457: 1-2ul; Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

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Related Product Information for BpiI restriction enzyme

5'-G A A G A C (N)2v-3'
3'-C T T C T G (N)6^-5'

Product Categories/Family for BpiI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

NCBI and Uniprot Product Information

• NCBI GI #: 408407672
• NCBI Official Full Name: Basic phospholipase A2 BP-II

Uniprot Description

Snake venom phospholipase A2 (PLA2) that shows anticoagulant activities, strong myolytic activity, infiltration of polymorphonuclear cells, and edema in stromal tissues. Induces cell death of Jurkat cells in a concentration dependent manner. Shows a low phospholipase A2 activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.