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Granulocyte Macrophage-Colony Stimulating Factor Active Protein | pGMCSF active protein

Recombinant Porcine Granulocyte Macrophage-Colony Stimulating Factor

Gene Names
Csf2; Gmcsf; Gm-csf
Purity
>95.0% as determined by SDS-PAGE.
Synonyms
Granulocyte Macrophage-Colony Stimulating Factor; Recombinant Porcine Granulocyte Macrophage-Colony Stimulating Factor; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim; GMCSF Porcine; Granulocyte Macrophage-Colony Stimulating Factor Porcine Recombinant; pGMCSF active protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>95.0% as determined by SDS-PAGE.
Form/Format
Sterile filtered white lyophilized (freeze-dried) powder.
The protein was lyophilized from a sterile (0.2um) filtered solution containing 10mM sodium phosphate, pH 7.5.
Sequence
Amino acid: MAPTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK
Sequence Length
144
Solubility
It is recommended to reconstitute the lyophilized GMCSF in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50, as determined by TF-1 cell proliferation is 4.52ng/ml corresponding to a specific activity which is 2.2 x 10^5 units/mg.
Preparation and Storage
Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C.
Upon reconstitution GMCSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.
Please prevent freeze-thaw cycles.
Related Product Information for pGMCSF active protein
Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of GMCSF is found extracellularly as a homodimer. GMCSF has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Description: Granulocyte Macrophage-Colony Stimulating Factor Porcine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 128 amino acids and having a molecular mass of 14.5kDa. GMCSF is purified by proprietary chromatographic techniques.
Product Categories/Family for pGMCSF active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2
NCBI Official Symbol
Csf2
NCBI Official Synonym Symbols
Gmcsf; Gm-csf
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
Csf2
UniProt Synonym Gene Names
Csfgm; GM-CSF; CSF
UniProt Entry Name
CSF2_RAT

NCBI Description

plays a role in alveolar epithelial fluid transport [RGD, Feb 2006]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space

Molecular Function: growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; positive regulation of interleukin-23 production; myeloid dendritic cell differentiation; regulation of cell proliferation; epithelial cell proliferation; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of gene expression; positive regulation of cell proliferation; immune response; positive regulation of macrophage differentiation; positive regulation of DNA replication

Research Articles on pGMCSF

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Product Notes

The pGMCSF csf2 (Catalog #AAA141479) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Amino acid: MAPTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK. It is sometimes possible for the material contained within the vial of "Granulocyte Macrophage-Colony Stimulating Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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