Phosphoglycerate Mutase 1 Active Protein | PGAM1 active protein
Recombinant Mouse Phosphoglycerate Mutase 1, Active
Gene Names
Pgam1; Pgam-1; 2310050F24Rik
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Phosphoglycerate Mutase 1; Recombinant Mouse Phosphoglycerate Mutase 1; Active; PGAM1 Mouse; Phosphoglycerate mutase 1; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B; Pgam1; Pgam-1; 2310050F24Rik; Phosphoglycerate Mutase 1 Mouse Recombinant; PGAM1 active protein
Host
E Coli
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
Sterile filtered clear colorless solution.
The PGAM1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
The PGAM1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK
Sequence Length
254
Biological Activity
Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Related Product Information for PGAM1 active protein
Description: PGAM1 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 278 amino acids (1-254) and having a molecular mass of 31.4kDa.PGAM1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: PGAM1 is part of the phosphoglycerate mutase family. PGAM1 is an essential component of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. PGAM1 is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). PGAM1 mutations lead to muscle phosphoglycerate mutase deficiency, a.k.a. glycogen storage disease X.
Introduction: PGAM1 is part of the phosphoglycerate mutase family. PGAM1 is an essential component of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. PGAM1 is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). PGAM1 mutations lead to muscle phosphoglycerate mutase deficiency, a.k.a. glycogen storage disease X.
Product Categories/Family for PGAM1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,832 Da
NCBI Official Full Name
phosphoglycerate mutase 1
NCBI Official Synonym Full Names
phosphoglycerate mutase 1
NCBI Official Symbol
Pgam1
NCBI Official Synonym Symbols
Pgam-1; 2310050F24Rik
NCBI Protein Information
phosphoglycerate mutase 1
UniProt Protein Name
Phosphoglycerate mutase 1
Protein Family
UniProt Gene Name
Pgam1
UniProt Synonym Gene Names
PGAM-B
Uniprot Description
PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.13; EC 5.4.2.11; EC 5.4.2.4; Isomerase; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 19|19 C3
Cellular Component: cytoplasm; cytosol; membrane; myelin sheath; nucleus
Molecular Function: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity; phosphoglycerate mutase activity; protein kinase binding
Biological Process: gluconeogenesis; glycolysis; regulation of glycolysis; regulation of pentose-phosphate shunt; respiratory burst
Research Articles on PGAM1
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Product Notes
The PGAM1 pgam1 (Catalog #AAA146827) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MGSHMA AYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK. It is sometimes possible for the material contained within the vial of "Phosphoglycerate Mutase 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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