NOGGIN active protein

Mouse NOGGIN

Cat. Num. AAA692050

• Reactivity: Mouse
• Purity: > 95% by SDS-PAGE
• Synonyms: NOGGIN; Mouse NOGGIN; Recombinant Murine NOGGIN; NOGGIN active protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Lyophilized
• Sequence: MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGG HYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM P SEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWND LGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQ RRGGQRCG WIPIQYPIIS ECKCSC
• Sequence Length: 232

• Endotoxin Level: < 0.1 ng per ug of NOGGIN
• Biological Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/ml of Noggin.

Related Product Information for NOGGIN active protein

Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-beta family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-beta ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.

Product Categories/Family for NOGGIN active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7110675
• NCBI GeneID: 18121
• NCBI Accession #: NP_032737.1
• NCBI GenBank Nucleotide #: NM_008711.2
• UniProt Accession #: P97466
• Molecular Weight: 46.4 kDa
• NCBI Official Full Name: noggin
• NCBI Official Synonym Full Names: noggin
• NCBI Official Symbol: Nog
• NCBI Protein Information: noggin
• UniProt Protein Name: Noggin
• Protein Family: Noggin
• UniProt Gene Name: Nog
• UniProt Entry Name: NOGG_MOUSE

Uniprot Description

Function: Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Ref.2

Subunit structure: Homodimer

By similarity.

Subcellular location: Secreted.

Tissue specificity: Expressed in condensing cartilage and immature chondrocytes.

Developmental stage: Embryonic expression was first detected in the node at 7.5 dpc. By early somite stages, expression extends anteriorly along the entire length of the notochord and is expressed in the dorsal neural tube from the caudal hindbrain to the posterior-most region of the embryo. By the time cranial tube closure is completed expression is continuous along most of the dorsal midline of the neural tube, to its rostral termination at the base of the forebrain. Expression in the neural tube and caudal notochord remains unchanged during early organogenesis from 9.5 dpc to 10.5 dpc.

Involvement in disease: Defects in Nog are the cause of a recessive lethal phenotype at birth. Multiple defects include a failure of neural tube closure, broad club-shaped limbs, loss of caudal vertebrae, a shortened body axis, and retention of a small vestigial tail.

Sequence similarities: Belongs to the noggin family.