IL-33 active protein

Mouse IL-33

Cat. Num. AAA691996

• Gene Names: Il33; Il-33; Il1f11; NF-HEV; 9230117N10Rik
• Reactivity: Mouse
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Synonyms: IL-33; Mouse IL-33; NF-HEV; IL-33 active protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: > 98% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQD QVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK D YSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQ LAL VEEKDESCNN IMFKLSKI
• Sequence Length: 266

• Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is <= 0.5 ng/ml, corresponding to a specific activity of >= 2 x 106units/mg.

Related Product Information for IL-33 active protein

IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Recombinant murine IL-33 is a 17.5 kDa protein containing 158 amino acid residues.

Product Categories/Family for IL-33 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 257900495
• NCBI GeneID: 77125
• NCBI Accession #: NP_001158196.1
• NCBI GenBank Nucleotide #: NM_001164724.1
• UniProt Accession #: Q8BVZ5; Q2YEJ4; Q3TQN0; Q99L46
• Molecular Weight: 29,991 Da
• NCBI Official Full Name: interleukin-33
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: Il33
• NCBI Official Synonym Symbols: Il-33; Il1f11; NF-HEV; 9230117N10Rik
• NCBI Protein Information: interleukin-33; nuclear factor from high endothelial venules
• UniProt Protein Name: Interleukin-33
• UniProt Gene Name: Il33
• UniProt Synonym Gene Names: IL-33
• UniProt Entry Name: IL33_MOUSE

Uniprot Description

Function: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8

By similarity. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. Ref.1 Ref.4In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. Ref.1 Ref.4

Subunit structure: Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP

By similarity.

Subcellular location: Nucleus. Chromosome. Cytoplasmic vesicle › secretory vesicle

By similarity. Secreted. Note: Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent

By similarity. Ref.4

Domain: The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association

By similarity.

Post-translational modification: Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Caspase-mediated proteolysis, once thought to activate IL33, rather acts as a switch to dampen its activity. Proteolysis is not strictly required for biological activity

By similarity.

Miscellaneous: Intraperitoneal injections of IL-33 induce the expression of IL-4, IL-5, and IL-13 and lead to severe pathological changes in mucosal organs.

Sequence similarities: Belongs to the IL-1 family. Highly divergent.