Dot Blot (DB)
(Dot Blot: Unmodified and various modified cytosine DNA were probed Anti-5-Hydroxymethylcytosine (5hmC) (1:5000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system)
Rat 5-Hydroxymethylcytosine Monoclonal Antibody | anti-5hmC antibody
5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine) (FITC)
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation
Purity
Purified by Protein G Affinity Chromatography
Synonyms
5-Hydroxymethylcytosine; Monoclonal Antibody; 5-Hydroxymethylcytosine (5hmC; 5'OH methyl cytosine) (FITC); anti-5hmC antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
HMC-MA01
Specificity
Detects 5-hydroxymethyl cytosine, but not 5-methylcytosine or unmethylated cytosine. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-5hmC antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Immunogen
BSA-conjugated recombinant Hydroxymethylcytosine DNA.
Conjugate
FITC
Preparation and Storage
Store at -20 degree C.
Dot Blot (DB)
(Dot Blot: Unmodified and various modified cytosine DNA were probed Anti-5-Hydroxymethylcytosine (5hmC) (1:5000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system)
Dot Blot (DB)
(Dot Blot: Unmodified and various modified cytosine DNA were probed Anti-5-Hydroxymethylcytosine (5hmC) (1:5000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system)
Product Categories/Family for anti-5hmC antibody
References
Wossidlo, Mark, et al. (2011) Nat Commun, 2: 241 (2011)
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Product Notes
The 5hmC (Catalog #AAA6243601) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 5-Hydroxymethylcytosine can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the 5hmC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-Hydroxymethylcytosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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