C5a active protein

Mouse C5a

Cat. Num. AAA692546

• Reactivity: Mouse
• Purity: >= 98% by SDS-PAGE gel and HPLC analyses
• Synonyms: C5a; Mouse C5a; Complement Component 5a; Complement 5a; CPAMD4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5a active protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: >= 98% by SDS-PAGE gel and HPLC analyses
• Form/Format: Lyophilized
• Sequence: NLHLLRQKIE EQAAKYKHSV PKKCCYDGAR VNFYETCEER VARVTIGPLC IRAFNECCTI ANKIRKESPH KPVQLGR
• Sequence Length: 77; Length [aa]

• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml.; Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week.; For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at ?20 degree C to -80 degree C.
• Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug)
• Biological Activity: Measured by its ability to support adhesion of Human Umbilical Vein Endothelial Cells (HUVEC) to the Recombinant Murine C5a-coated surface.
• Preparation and Storage: The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months.; Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C, and for 3 months at -20 degree C to -80 degree C.

Related Product Information for C5a active protein

Complement 5a (C5a) is an enzymatically generated glycoprotein belonging to the anaphylatoxin family of structurally and functionally related proteins. Generated upon the activation of the complement system, C5a, together with C4a, C3a, and the membrane attack complex (C5b?9), functions as a central player in host defense by inducing smooth muscle cell contraction, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes through cell degranulation. In addition to acting as a direct mediator of localized inflammatory response, C5a also initiates both the synthesis and release of IL?8 from monocytes and bronchial epithelial cells, stimulates the proliferation of neurons and hepatocytes, and functions as a potent chemoattractant. Where C5a deficiency, a rare defect of the complement pathway caused by the mutation of the C5a gene, is associated with susceptibility to severe infections, excessive C5a activation has been linked to liver fibrosis, sepsis, adult respiratory distress syndrome, rheumatoid arthritis, Alzheimer’s disease, and ischemic heart disease. Human C5a shares 60% and 54% sequence identity to mouse and rat C5a, respectively. The human C5 gene encodes a 1,676 amino acid glycoprotein that is comprised of a disulfide?linked C5 alpha and a C5 beta chain, the former of which contains the active, 74 amino acid C5a anaphylatoxin chain. Recombinant Murine C5a is an 8.9 kDa glycoprotein containing the 77 amino acid residues of the C5a anaphylatoxin chain.

NCBI and Uniprot Product Information

• NCBI GI #: 6754164
• NCBI Accession #: NP_034536.1
• NCBI GenBank Nucleotide #: NM_010406.2
• UniProt Accession #: P06684
• Molecular Weight: 8.9 kDa
• NCBI Official Full Name: complement C5 preproprotein
• UniProt Protein Name: Complement C5
• Protein Family: C5a peptidase
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: Hc
• UniProt Entry Name: CO5_MOUSE

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: endopeptidase inhibitor activity

Biological Process: positive regulation of angiogenesis; in utero embryonic development; immune system process; cytolysis; complement activation, alternative pathway; innate immune response; inflammatory response; complement activation, classical pathway