Lactate Dehydrogenase A Active Protein | LDHA active protein

Recombinant Human Lactate Dehydrogenase A

Cat. Num. AAA143494

• Gene Names: LDHA; LDH1; LDHM; GSD11; PIG19; HEL-S-133P
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Lactate Dehydrogenase A; Recombinant Human Lactate Dehydrogenase A; LDHA Human; Lactate Dehydrogenase A Human Recombinant; LDH-A; GSD11; LDH1; LDHM; PIG19; EC 1.1.1.27; lactate dehydrogenase M; LDH-M; LDH-1; L-lactate dehydrogenase A chain; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; Cell proliferation-inducing gene 19 protein; LDHA; LDHA active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The LDHA protein solution contains 20mM Tris-HCl pH-8, 100mM NaCl and 20% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
• Sequence Length: 274

• Biological Activity: Specific activity is > 20 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37 degree C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for LDHA active protein

Description: LDHA Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (1-332 a.a.) and having a molecular mass of 38.8 kDa. The LDHA is fused to a 20 amino acid his tag at N-terminus and purified by conventional chromatography.

Introduction: LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

Product Categories/Family for LDHA active protein

Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 207028494
• NCBI GeneID: 3939
• NCBI Accession #: NP_001128711.1
• NCBI GenBank Nucleotide #: NM_001135239.1
• UniProt Accession #: P00338; Q53G53; Q6IBM7; Q6ZNV1; Q9UDE8; Q9UDE9; B4DKQ2; B7Z5E3; D3DQY3; F8W819
• Molecular Weight: 26,712 Da
• NCBI Official Full Name: L-lactate dehydrogenase A chain isoform 2
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: LDHA
• NCBI Official Synonym Symbols: LDH1; LDHM; GSD11; PIG19; HEL-S-133P
• NCBI Protein Information: L-lactate dehydrogenase A chain; LDH muscle subunit; LDH-A; LDH-M; cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; lactate dehydrogenase M; renal carcinoma antigen NY-REN-59
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHA
• UniProt Synonym Gene Names: LDH-A; LDH-M
• UniProt Entry Name: LDHA_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Amino Acid Metabolism - cysteine and methionine; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.1.1.27; Carbohydrate Metabolism - propanoate; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: membrane; mitochondrion; nucleus; cytosol

Molecular Function: protein binding; L-lactate dehydrogenase activity

Biological Process: cellular metabolic process; substantia nigra development; glycolysis; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process

Disease: Glycogen Storage Disease Xi