Lactate Dehydrogenase A Active Protein | LDHA active protein
Recombinant E Coli Lactate Dehydrogenase A, BioActive
Gene Names
ldhA; ECK1377; hslF; hslI; htpH; JW1375
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Lactate Dehydrogenase A; Recombinant E Coli Lactate Dehydrogenase A; BioActive; LDHA Active; LDHA; E Coli Active; BioActive E Coli Recombinant; LDH-A; GSD11; LDH1; LDHM; PIG19; EC 11127; lactate dehydrogenase M; LDH-M; LDH-1; L-lactate dehydrogenase A chain; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; Cell proliferation-inducing gene 19 protein; LDHA active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
Sterile filtered colorless solution.
LDHA E Coli protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), containing 100mM NaCland 10% glycerol.
LDHA E Coli protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), containing 100mM NaCland 10% glycerol.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV
Biological Activity
Specific activity is >200unit/mg. 1 unit converts 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH7.5 at 37 degree C
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Related Product Information for LDHA active protein
D-lactate dehydrogenase or ldhA is an enzyme, part of the D-lactate dehydrogenase protein family. IDHA is a cytochrome that enhances the catalyzation of D-lactate dehydrogenase ldhA reaction. This enzyme has 2 substrates ((D) -lactate & ferricytochrome c), thus, it has 2 end products (pyruvate & ferrocytochrome c).
LDHA E Coli Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 353 amino acids (1-329) and having a molecular mass of 39.1 kDa.
LDHA E Coli is fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
LDHA E Coli Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 353 amino acids (1-329) and having a molecular mass of 39.1 kDa.
LDHA E Coli is fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Categories/Family for LDHA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,535 Da
NCBI Official Full Name
fermentative D-lactate dehydrogenase, NAD-dependent
NCBI Official Symbol
ldhA
NCBI Official Synonym Symbols
ECK1377; hslF; hslI; htpH; JW1375
NCBI Protein Information
fermentative D-lactate dehydrogenase, NAD-dependent
UniProt Protein Name
D-lactate dehydrogenase
Protein Family
UniProt Gene Name
ldhA
UniProt Synonym Gene Names
hslI; htpH; D-LDH
UniProt Entry Name
LDHD_ECOLI
NCBI Description
There are three lactate dehydrogenase enzymes in Escherichia coli which interconvert pyruvate and lactate. [More information is available at EcoCyc: G592].
Uniprot Description
Fermentative lactate dehydrogenase.
Research Articles on LDHA
Similar Products
Product Notes
The LDHA ldha (Catalog #AAA141589) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV. It is sometimes possible for the material contained within the vial of "Lactate Dehydrogenase A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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