Interleukin 10 (mIL 10) Active Protein | IL-10 active protein
Mouse interleukin 10 (mIL 10)
Purity
Purity: Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
Interleukin 10 (mIL 10); Mouse interleukin 10 (mIL 10); IL-10 active protein
Host
E Coli
Purity/Purification
Purity: Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated ( ~ 1mg/ml) solution containing 0.02-0.03% of NaHCO3 and glycine.
Sequence
The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Ser-Pro-Gly-Gln.
Solubility
Lyophilized mouse interleukin 10 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL10 should be stored sterile at 4 degree C between 7-14 days and for future use below -18 degree C. Please avoid freeze-thaw cycles.
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.430 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified hIL 10 consists of > 90% dimers as determined by gel-filtration chromatography under non reducing conditions. However about 25% of the dimers are covalent.
Biological Activity
mIL 10 is fully biologically active as evidenced by its ability to induce proliferation of IM/9 cells in presence of IL 3.
Endotoxin
Less than 0.0025 ng/ug (0.025 IEU/ug) of mIL 10.
Preparation and Storage
It is recommended to reconstitute the lyophilized mIL 10 in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein such as BSA or HSA.
Related Product Information for IL-10 active protein
Mouse IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in inhibition of immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Mouse recombinant Interleukin-10 produced in E. coli is a non covalent dimer of two polypeptide chains containing 161 amino acids each and having a molecular mass of 18 kDa. Mouse IL-10 is purified by proprietary cinematographic techniques.
Mouse recombinant Interleukin-10 produced in E. coli is a non covalent dimer of two polypeptide chains containing 161 amino acids each and having a molecular mass of 18 kDa. Mouse IL-10 is purified by proprietary cinematographic techniques.
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Product Notes
The IL-10 (Catalog #AAA400138) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Ser-Pr o-Gly-Gln. It is sometimes possible for the material contained within the vial of "Interleukin 10 (mIL 10), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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