WISP-1 active protein

Human WISP-1

Cat. Num. AAA695951

• Gene Names: WISP1; CCN4; WISP1c; WISP1i; WISP1tc
• Reactivity: Species Reactivity: Human; Cross Reactivity: Mouse, Rat
• Purity: Above 98% as determined by SDS-PAGE Analysis.
• Synonyms: WISP-1; Human WISP-1; Recombinant Human WISP-1; WISP-1 active protein

• Host: E Coli
• Reactivity: Species Reactivity: Human; Cross Reactivity: Mouse, Rat
• Purity/Purification: Above 98% as determined by SDS-PAGE Analysis.
• Sequence: TALSPAPTT MDFTPAPLE DTSSRPQFC KWPCECPPS PPRCPLGVS LITDGCECC KMCAQQLGD NCTEAAICD PHRGLYCDY SGDRPRYAI GVCAQVVGV GCVLDGVRY NNGQSFQPN CKYNCTCID GAVGCTPLC LRVRPPRLW CPHPRRVSI PGHCCEQWV CEDDAKRPR KTAPRDTGA FDAVGEVEA WHRNCIAYT SPWSPCSTS CGLGVSTRI SNVNAQCWP EQESRLCNL RPCDVDIHT LIKAGKKCL AVYQPEASM NFTLAGCIS TRSYQPKYC GVCMDNRCC IPYKSKTID VSFQCPDGL GFSRQVLWI NACFCNLSC RNPNDIFAD LESYPDFSE IAN

• Biological Activity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml.
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for WISP-1 active protein

WISP-1 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is expressed in the heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. WISP-1 is a beta catenin regulated protein that can contribute to tumorigenesis and has also been shown to play a role in bone development and fracture repair. Human WISP-1 is a 38.1 kDa protein containing 346 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain.

Product Categories/Family for WISP-1 active protein

Cancer; Bone Biology

NCBI and Uniprot Product Information

• NCBI GI #: 34098393
• NCBI GeneID: 8840
• UniProt Accession #: O95388; Q5JBS6; Q5JBS7; Q5JBS8; Q9HCS3; A8KAG6; E7EMM5
• Molecular Weight: 21,550 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 1
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 1
• NCBI Official Symbol: WISP1
• NCBI Official Synonym Symbols: CCN4; WISP1c; WISP1i; WISP1tc
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 1; CTC-458A3.8; CCN family member 4; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 1
• UniProt Gene Name: WISP1
• UniProt Synonym Gene Names: CCN4; WISP-1
• UniProt Entry Name: WISP1_HUMAN

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Oncoprotein; Secreted

Chromosomal Location of Human Ortholog: 8q24.22

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding

Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction