WISP-3 active protein

Human WISP-3

Cat. Num. AAA691873

• Gene Names: WISP3; PPD; CCN6; LIBC; PPAC
• Reactivity: Human
• Purity: 98 % (SDS-PAGE, HPLC)
• Synonyms: WISP-3; Human WISP-3; Recombinant Human WISP-3; WISP-3 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: 98 % (SDS-PAGE, HPLC)
• Form/Format: Lyophilized
• Sequence: TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVR DGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV C AYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKL AGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IW KKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQP CD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFC GICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCR E PGDIFSELKI L
• Sequence Length: 354

• Endotoxin Level: < 0.1 ng per ug of WISP-3
• Biological Activity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml.

Related Product Information for WISP-3 active protein

WISP-3 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is predominantly expressed in the adult kidney, testis and fetal kidney and weaker expression in placenta, ovary, prostate, small intestine, and skeletally-derived cells. WISP-3 is required for normal postnatal skeletal growth and cartilage homeostasis. Human WISP-3 is a 36.8 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cystine knot-like (CTCK) domain.

Product Categories/Family for WISP-3 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4507925
• NCBI GeneID: 8838
• NCBI Accession #: NP_003871.1
• NCBI GenBank Nucleotide #: NM_003880.3
• UniProt Accession #: O95389; Q3KR29; Q5H8W4; Q6UXH6
• Molecular Weight: 36.8 kDa
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 3 isoform 1
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 3
• NCBI Official Symbol: WISP3
• NCBI Official Synonym Symbols: PPD; CCN6; LIBC; PPAC
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 3; WISP-3; CCN family member 6
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 3
• UniProt Gene Name: WISP3
• UniProt Synonym Gene Names: CCN6; WISP-3
• UniProt Entry Name: WISP3_HUMAN

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Appears to be required for normal postnatal skeletal growth and cartilage homeostasis.

Subcellular location: Secreted

Probable.

Tissue specificity: Predominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes.

Involvement in disease: Progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]: Autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.6

Sequence similarities: Belongs to the CCN family.Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 1 IGFBP N-terminal domain.Contains 1 TSP type-1 domain.