WISP3 active protein

Recombinant Human WISP3

Cat. Num. AAA4159864

• Gene Names: CCN6; PPD; LIBC; PPAC; WISP3; WISP-3
• Purity: >95% as determined by SDS-PAGE
• Synonyms: WISP3; Recombinant Human WISP3; WNT1-inducible-signaling pathway protein 3; WISP3 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized Powder
• Sequence Length: 354

• Bioactivity: The ED(50) was determined by the dose-dependent proliferation of MCF7 cells and was found to be <0.5ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for WISP3 active protein

WNT1-inducible-signaling pathway protein 3 (WISP3) is a member of the CCN family of cysteine-rich, glycosylated signalling proteins that mediate several developmental processes, and a member of the WNT1 inducible signalling pathway (WISP) subfamily. Members of this family are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain (IGFBP), von Willebrand factor type C (VWFC) domain, thrombospondin type-1 repeat (TSP type-1) domain, and C-terminal cystine knot-like (CTCK) domain. WISP3 is expressed in mature and fetal kidneys, testis, and in lower levels in the ovary, placenta, prostate, and small intestine. It is necessary for the growth of postnatal skeleton and cartilage homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 4507925
• NCBI GeneID: 8838
• NCBI Accession #: NP_003871.1
• NCBI GenBank Nucleotide #: NP_003871.1
• UniProt Accession #: O95389
• Molecular Weight: 37.1 kDa
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 3 isoform 1
• NCBI Official Synonym Full Names: cellular communication network factor 6
• NCBI Official Symbol: CCN6
• NCBI Official Synonym Symbols: PPD; LIBC; PPAC; WISP3; WISP-3
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 3
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 3
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP3
• UniProt Synonym Gene Names: CCN6; WISP-3
• UniProt Entry Name: WISP3_HUMAN

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WISP3: Appears to be required for normal postnatal skeletal growth and cartilage homeostasis. Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC). PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: proteinaceous extracellular matrix; extracellular space

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion

Disease: Arthropathy, Progressive Pseudorheumatoid, Of Childhood