HB-EGF active protein

Human HB-EGF

Cat. Num. AAA691493

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Reactivity: Human
• Purity: > 95% by SDS-PAGE and HPLC analysis
• Synonyms: HB-EGF; Human HB-EGF; Recombinant Human HB-EGF; HB-EGF active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized
• Sequence: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPC LRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
• Sequence Length: 208

• Endotoxin Level: < 0.1 ng per ug of HB-EGF
• Biological Activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is <= 1.0 ng/ml, corresponding to a specific activity of >= 1 x 106 units/mg.

Related Product Information for HB-EGF active protein

HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.

Product Categories/Family for HB-EGF active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4503413
• NCBI GeneID: 1839
• NCBI Accession #: NP_001936.1
• NCBI GenBank Nucleotide #: NM_001945.2
• UniProt Accession #: Q99075; B2R821
• Molecular Weight: 9.7 kDa
• NCBI Official Full Name: proheparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL; HB-EGF; HBEGF; DT-R
• UniProt Entry Name: HBEGF_HUMAN

Uniprot Description

Function: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Subunit structure: Interacts with FBLN1

By similarity. Interacts with EGFR and ERBB4. Ref.9

Subcellular location: Heparin-binding EGF-like growth factor: Secreted › extracellular space. Note: Mature HB-EGF is released into the extracellular space and probably binds to a receptor.Proheparin-binding EGF-like growth factor: Cell membrane; Single-pass type I membrane protein.

Post-translational modification: Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.O-glycosylated with core 1 or possibly core 8 glycans. Thr-47 is a minor glycosylation site compared to Thr-44. Ref.7 Ref.11 Ref.12

Sequence similarities: Contains 1 EGF-like domain.