Human Cytochrome P450 1B1 (CYP1B1) RTU ELISA Kit | CYP1B1 rtu elisa kit

Human Cytochrome P450 1B1 (CYP1B1) ELISA Kit

Cat. Num. AAA458734

• Gene Names: CYP1B1; CP1B; ASGD6; GLC3A; CYPIB1; P4501B1
• Reactivity: Human
• Synonyms: Cytochrome P450 1B1 (CYP1B1); Human Cytochrome P450 1B1 (CYP1B1) ELISA Kit; CP1B; GLC3A; Cytochrome P450; Family 1; Subfamily B; Polypeptide 1; Glaucoma 3; Primary Infantile; CYP1B1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 543

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.063ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP1B1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP1B1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYP1B1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP1B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CYP1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 189491763
• NCBI GeneID: 1545
• NCBI Accession #: NP_000095.2
• NCBI GenBank Nucleotide #: NM_000104.3
• UniProt Accession #: Q16678; Q5TZW8; Q93089; Q9H316
• Molecular Weight: 60,846 Da
• NCBI Official Full Name: cytochrome P450 1B1
• NCBI Official Synonym Full Names: cytochrome P450 family 1 subfamily B member 1
• NCBI Official Symbol: CYP1B1
• NCBI Official Synonym Symbols: CP1B; ASGD6; GLC3A; CYPIB1; P4501B1
• NCBI Protein Information: cytochrome P450 1B1
• UniProt Protein Name: Cytochrome P450 1B1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1B1

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.