HB-EGF Active Protein | HBEGF active protein

HB-EGF Protein, Human, Recombinant

Cat. Num. AAA8120191

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Purity: >95% as determined by SDS-PAGE
• Synonyms: HB-EGF; HB-EGF Protein; Human; Recombinant; Human HBEGF/DTR Protein; DTR Protein; DTS Protein; DTSF Protein; HEGFL Protein; heparin-binding EGF-like growth factor; HBEGF active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu148

• Species: Human
• Activity: Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED₅₀ for this effect is typically 0.4-2ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 63
• Tag: Native
• Protein Construction: A DNA sequence encoding the human HBEGF (Q99075)(Met 1-Leu 148) without the C-terminal propeptide (aa 149-208), was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 0.4-2ng/mL.)

Related Product Information for HBEGF active protein

Background: Heparin-binding EGF-like growth factor (HBEGF), a member of the EGF family of growth factors, exerts its biological activity through activation of the EGFR and other ErbB receptors. Soluble mature HBEGF is proteolytically processed from a larger membrane-anchored precursor and is a potent mitogen and chemotactic factor for fibroblasts, smooth muscle cells but not endothelial cells. HBEGF activates two EGF receptor subtypes, HER1 and HER4, and binds to cell surface HSPG. The transmembrane form of HBEGF is a juxtacrine growth and adhesion factor and is uniquely the receptor for diphtheria toxin. Both forms of HB-EGF participate in normal physiological processes and pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HBEGF participates in diverse biological processes, including heart development and maintenance, skin wound healing, eyelid formation, blastocyst implantation, the progression of atherosclerosis, and tumor formation, through the activation of signaling molecules downstream of ErbB receptors and interactions with molecules associated with HBEGF. tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta markedly increased HB-EGF mRNA levels in HUVEC by 12- and 7-fold, respectively, and induction of the gene by TNF-alpha was both dose- and time-dependent.

References

Miyamoto S, et al. (2006) Heparin-binding epidermal growth factor-like growth factor as a novel targeting molecule for cancer therapy. Cancer Sci. 97(5): 341-7.Raab G, et al. (1997) Heparin-binding EGF-like growth factor. Biochim Biophys Acta. 1333(3): 179-99.Pathak BG, et al. (1995) Mouse chromosomal location of three EGF receptor ligands: amphiregulin (Areg), betacellulin (Btc), and heparin-binding EGF (Hegfl). Genomics. 28(1): 116-8.

NCBI and Uniprot Product Information

• NCBI GI #: 4503413
• NCBI GeneID: 1839
• NCBI Accession #: NP_001936.1
• NCBI GenBank Nucleotide #: NM_001945.2
• UniProt Accession #: Q99075; B2R821
• Molecular Weight: 23,067 Da
• NCBI Official Full Name: proheparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• Protein Family: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL; HB-EGF; HBEGF; DT-R
• UniProt Entry Name: HBEGF_HUMAN

Uniprot Description

HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; negative regulation of elastin biosynthetic process; positive regulation of smooth muscle cell proliferation; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; wound healing, spreading of epidermal cells; positive regulation of cell migration