thrombopoietin Active Protein | hTPO active protein

Human thrombopoietin (hTPO)

Cat. Num. AAA400073

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Anion-exchange FPLC.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
• Synonyms: thrombopoietin; Human thrombopoietin (hTPO); hTPO active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Anion-exchange FPLC.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
• Form/Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Ala-Pr-Pro.
• Sequence Length: 353

• Solubility: It is recommended to reconstitute the lyophilized Recombinant TPO in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm.; 2. Analysis by RP-HPLC, using a calibrated solution of TPO as a Reference Standard.
• Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of TPO.
• Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
• Biological Activity: Recombinant TPO is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of MO7e cells is less then 1 ng/ml, corresponding to a Specific Activity of 1 x 10 6 IU/mg.
• Preparation and Storage: Lyophilized Recombinant TPO although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Recombinant TPO should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please avoid freeze-thaw cycles.

Related Product Information for hTPO active protein

Recombinant Human Thrombopoietin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a molecular mass of 18608 Dalton which comprises the receptor binding domain of the Mpl-ligand protein.
Recombinant Human Thrombopoietin is purified by proprietary chromatographic techniques.

Product Categories/Family for hTPO active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1401248
• NCBI GeneID: 7066
• UniProt Accession #: P40225; Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8
• Molecular Weight: 33,604 Da
• NCBI Official Full Name: thrombopoietin
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• NCBI Protein Information: thrombopoietin; MPL ligand; c-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: THPO
• UniProt Synonym Gene Names: MGDF; ML; MGDF
• UniProt Entry Name: TPO_HUMAN

NCBI Description

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: growth factor activity; hormone activity; cytokine activity

Biological Process: platelet activation; cell proliferation; positive regulation of protein kinase B signaling cascade; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1