interleukin 10 Active Protein | hIL 10 active protein

Human interleukin 10

Cat. Num. AAA400019

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Purity: Purity:Greater than 98.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Synonyms: interleukin 10; Human interleukin 10; Human interleukin 10 (hIL 10); hIL 10 active protein

• Host: E Coli
• Purity/Purification: Purity:Greater than 98.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Form/Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Ser-Pro-Gly-Gln.
• Sequence Length: 55

• Solubility: Lyophilized Interleukin 10 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL10 should be stored sterile at 4 degree C between 7-14 days and for future use below -18 degree C.; Please avoid freeze-thaw cycles.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.46 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: The purified hIL 10 consists of > 99% dimers as determined by gel-filtration chromatography under non reducing conditions.
• Endotoxin: Less than 0.05 ng/ug (0.5 IEU/ug) of hIL 10
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution containing 0.02-0.03% of NaHCO3 and glycine.
• Biological Activity: hIL 10 is fully biologically active as evidenced by its ability to induce proliferation of IM/9 cells in presence of IL 3.
• Preparation and Storage: It is recommended to reconstitute the lyophilized hIL 10 in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein such as BSA or HSA.

Related Product Information for hIL 10 active protein

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in inhibition of immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Interleukin-10 Human Recombinant produced in E. coli is a non covalent dimer of two polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton.
The IL-10 is purified by proprietary chromatographic techniques.

Product Categories/Family for hIL 10 active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 14625940
• NCBI GeneID: 3586
• UniProt Accession #: P22301
• Molecular Weight: 6,026 Da
• NCBI Official Full Name: interleukin-10, partial
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: IL10
• UniProt Entry Name: Q6LBF4_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Motility/polarity/chemotaxis; Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding

Biological Process: negative regulation of chronic inflammatory response to antigenic stimulus; B cell proliferation; regulation of isotype switching; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; cytoplasmic sequestering of NF-kappaB; negative regulation of T cell proliferation; cell-cell signaling; negative regulation of interferon-gamma production; response to molecule of bacterial origin; positive regulation of MHC class II biosynthetic process; negative regulation of interleukin-6 production; hemopoiesis; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; T-helper 2 type immune response; positive regulation of B cell apoptosis; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to activity; receptor biosynthetic process; regulation of sensory perception of pain; positive regulation of cytokine secretion; negative regulation of apoptosis

Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To