Epidermal Growth Factor Active Protein | EGF active protein

Recombinant Mouse Epidermal Growth Factor, Biotin

Cat. Num. AAA141171

• Gene Names: Egf; AI790464
• Purity: Greater than 95.0% as determined by analysis by SDS-PAGE.
• Synonyms: Epidermal Growth Factor; Recombinant Mouse Epidermal Growth Factor; Biotin; EGF Mouse; Epidermal Growth Factor Mouse Recombinant; Urogastrone; URG; EGF; EGF active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by analysis by SDS-PAGE.
• Form/Format: The protein (0.5 mg/ml) solution contains sterile PBS.; Sterile Filtered clear solution.
• Sequence: MKKIDDDKNS YPGCPSSYDG YCLNGGVCMH IESLDSYTCN CVIGYSGDRC QTRDLEWWEL R.
• Sequence Length: 1217

• Biological Activity: The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 0.1ng/ml corresponding to a specific activity of 10,000,000units/mg.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Related Product Information for EGF active protein

Introduction: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description: EGF Mouse Recombinant, Biotin produced in E Coli is a non-glycosylated polypeptide chain containing 61 amino acids and having a total molecular mass of 7.0kDa. This version of EGF has a N terminal leader sequence hosting a biotin conjugation. There are 0.5 biotins for each EGF protein.

Product Categories/Family for EGF active protein

Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 170172524
• NCBI GeneID: 13645
• NCBI Accession #: NP_034243.2
• NCBI GenBank Nucleotide #: NM_010113.4
• UniProt Accession #: P01132; Q569W5; Q6P9J2; E9QNX6
• Molecular Weight: 133,072 Da
• NCBI Official Full Name: pro-epidermal growth factor isoform 1
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: Egf
• NCBI Official Synonym Symbols: AI790464
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: Epidermal growth factor
• UniProt Gene Name: Egf
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_MOUSE

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Ligand, receptor tyrosine kinase; Membrane protein, integral

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; receptor complex

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding; protein-tyrosine kinase activity; Wnt receptor activity; Wnt-protein binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; angiogenesis; branching morphogenesis of a tube; epidermal growth factor receptor signaling pathway; negative regulation of secretion; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor activity; positive regulation of fibroblast proliferation; positive regulation of granule cell precursor proliferation; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; regulation of peptidyl-tyrosine phosphorylation; regulation of protein secretion; regulation of protein transport; STAT protein nuclear translocation; Wnt receptor signaling pathway through beta-catenin