Rabbit anti-Human LPL Polyclonal Antibody | anti-LPL antibody

LPL, ID (LPL, LIPD, Lipoprotein lipase) (FITC)

Cat. Num. AAA6316815

• Gene Names: LPL; LIPD; HDLCQ11
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: LPL; Polyclonal Antibody; ID (LPL; LIPD; Lipoprotein lipase) (FITC); Lipoprotein lipase; anti-LPL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LPL.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LPL antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 300-327 in the central region of human LPL, UniProt Accession #P06858. Species sequence homology: bovine, mouse, porcine and rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS6002431. This demonstrates that MBS6002431 detected the LPL protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6002431 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002431 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells (right histogram) using MBS6002431 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LPL antibody

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq].

Product Categories/Family for anti-LPL antibody

Antibodies; Enzymes; Lipase

References

1. Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). 2. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 3. Johansen, C.T., et al. Nat. Genet. 42(8):684-687(2010). 4. Zabaneh, D., et al. PLoS ONE 5 (8) (2010). 5. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4557727
• NCBI GeneID: 4023
• NCBI Accession #: NP_000228.1
• NCBI GenBank Nucleotide #: NM_000237.2
• UniProt Accession #: P06858; Q16282; Q16283; Q96FC4; B2R5T9
• Molecular Weight: 53,162 Da
• NCBI Official Full Name: lipoprotein lipase
• NCBI Official Synonym Full Names: lipoprotein lipase
• NCBI Official Symbol: LPL
• NCBI Official Synonym Symbols: LIPD; HDLCQ11
• NCBI Protein Information: lipoprotein lipase
• UniProt Protein Name: Lipoprotein lipase
• Protein Family: Lipoprotein
• UniProt Gene Name: LPL
• UniProt Synonym Gene Names: LIPD; LPL
• UniProt Entry Name: LIPL_HUMAN

NCBI Description

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Membrane protein, GPI anchor; EC 3.1.1.34; Lipid Metabolism - glycerolipid; Phospholipase

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; plasma membrane; extracellular region

Molecular Function: heparin binding; triacylglycerol lipase activity; protein binding; lipoprotein lipase activity; apolipoprotein binding; phospholipase activity; receptor binding; triglyceride binding

Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; lipoprotein metabolic process; triacylglycerol biosynthetic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process

Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined