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Uracil-DNA Glycosylase (UDG) DNA Repair Protein | UDG dna repair protein

Uracil-DNA Glycosylase (UDG)

Applications
Eliminates PCR Carry-Over Contamination
Synonyms
Uracil-DNA Glycosylase (UDG); UDG dna repair protein
Ordering
For Research Use Only!
Host
E Coli
Form/Format
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol, pH 7.4 at 25 degree C
Concentration
5,000 U/ml (varies by lot)
Applicable Applications for UDG dna repair protein
Eliminates PCR Carry-Over Contamination
Source Note
An E Coli strain that carries the UDG gene from E Coli.
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 ul reaction containing 0.2 ug DNA (104-105 cpm/ug) in 30 minutes at 37 degree C.
Reaction Conditions
1X UDG Reaction Buffer, Incubate at 37 degree C
Reaction Buffer or Mix
1X UDG Reaction Buffer:
20 mM Tris-HCl, 1 mM DTT, 1 mM EDTA, pH 8 at 25 degree C
Heat Inactivation
No
Preparation and Storage
Store at -20 degree C.
Related Product Information for UDG dna repair protein
E.coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases). It releases uracil from ss- or ds-DNA and is applicable to eliminates PCR carry-over contamination.

Similar Products

Product Notes

The UDG (Catalog #AAA9141669) is a DNA Repair Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Uracil-DNA Glycosylase (UDG) can be used in a range of immunoassay formats including, but not limited to, Eliminates PCR Carry-Over Contamination. Researchers should empirically determine the suitability of the UDG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Uracil-DNA Glycosylase (UDG), DNA Repair Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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