DNA Polymerase I DNA Manipulation

DNA Polymerase I, Large (Klenow) Fragment

Cat. Num. AAA9141653

• Applications: Blunting, Polymerases for DNA Manipulation, DNA Sequencing, RT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
• Synonyms: DNA Polymerase I; Large (Klenow) Fragment; DNA Polymerase I dna manipulation

• Host: E Coli
• Form/Format: 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25 degree C
• Concentration: 5,000 U/ml (varies by lot)

• Applicable Applications for DNA Polymerase I dna manipulation: Blunting, Polymerases for DNA Manipulation, DNA Sequencing, RT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
• Source Note: An E Coli strain that contains the E Coli polA gene that has had its 5'-->3' exonuclease domain removed.
• Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 degree C.
• Reaction Conditions: 1X ABuffer B, Incubate at 25 degree C
• Reaction Buffer or Mix: 1X ABuffer B:; 10 mM Tris-HC, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH7.9 at 25 degree C
• Heat Inactivation: 75 degree C for 20 min
• 5' - 3' Exonuclease: No
• 3' - 5' Exonuclease: Yes
• Strand Displacement: +
• Error Rate: ~ 18x10^6 bases
• Preparation and Storage: Store at -20 degree C.

Related Product Information for DNA Polymerase I dna manipulation

DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'-->5' exonuclease activity, but has lost 5'-->3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini. It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5' overhangs to form blunt ends, removal of 3' overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.