cilliary neurothropic factor Active Protein | CNTF active protein

Human cilliary neurothropic factor (CNTF)

Cat. Num. AAA400005

• Gene Names: CNTF; HCNTF
• Purity: Purity:Greater than 99.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Synonyms: cilliary neurothropic factor; Human cilliary neurothropic factor (CNTF); Human cilliary neurothropic factor (hCNTF); CNTF active protein

• Host: E Coli
• Purity/Purification: Purity:Greater than 99.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first four N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr
• Sequence Length: 200

• Solubility: It is recommended to reconstitute the lyophilized hCNTF in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.242 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: The purified hCNTF (22K) consists of > 99% monomers as determined by gel-filtration chromatography.
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of hCNTF
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.
• Biological Activity: hCNTF is fully biologically active as evidenced by its ability to phosphorylate STAT3 in several cells lines.
• Preparation and Storage: Lyophilized hCNTF although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg/ml and up to 2 mg/ml mM and filter sterilization hCNTF can be stored at 4 degree C for several weeks.

Related Product Information for CNTF active protein

CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant human CNTF is synthesized as a 200 amino acid polypeptide (22.7 kDa) lacking a hydrophobic N-terminal signal for secretion.

Product Categories/Family for CNTF active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4758020
• NCBI GeneID: 1270
• NCBI Accession #: NP_000605.1
• NCBI GenBank Nucleotide #: NM_000614.3
• UniProt Accession #: P26441; B2RAB2
• Molecular Weight: 22,931 Da
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: CNTF
• NCBI Official Synonym Symbols: HCNTF
• NCBI Protein Information: ciliary neurotrophic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: CNTF
• UniProt Synonym Gene Names: CNTF
• UniProt Entry Name: CNTF_HUMAN

NCBI Description

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

Uniprot Description

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: interleukin-6 receptor binding; growth factor activity; ciliary neurotrophic factor receptor binding

Biological Process: negative regulation of photoreceptor cell differentiation; positive regulation of cell proliferation; neuron development; regulation of retinal cell programmed cell death; negative regulation of neuron apoptosis; signal transduction; muscle morphogenesis; positive regulation of tyrosine phosphorylation of Stat3 protein; growth