anti-Human APAF-1-ALT Antibody | anti-APAF-1-ALT antibody

APAF-1-ALT Antibody

Cat. Num. AAA853547

• Gene Names: APAF1; CED4; APAF-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: APAF-1-ALT; APAF-1-ALT Antibody; APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19; dJ179L10.2; likely ortholog of mouse monocyte macrophage 19; anti-APAF-1-ALT antibody

• Reactivity: Human
• Specificity: APAF-1-ALT antibody detects endogenous levels of total APAF-1-ALT protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1194

• Applicable Applications for anti-APAF-1-ALT antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human APAF-1-ALT.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using APAF-1-ALT antibody.)

Western Blot (WB)

(Western blot analysis of extracts from COLO205, using APAF-1-ALT antibody.)

References

Keigo Machida, J. Biol. Chem., Apr 2001; 276: 12140-12146. Liam O'Connor, Cancer Res., Mar 2000; 60: 1217-1220. Zeping Wang, Nucleic Acids Res., Apr 2005; 33: 2248-2258 Govindaraj Anumanthan, Cancer Res., Nov 2006; 66: 10824-10832.

NCBI and Uniprot Product Information

• NCBI GI #: 4502123
• NCBI GeneID: 317
• NCBI Accession #: NP_001151.1
• NCBI GenBank Nucleotide #: NM_001160.2
• UniProt Accession #: O14727; O43297; Q7Z438; Q9BXZ6; Q9UBZ5; Q9UGN8; Q9UGN9; Q9UGP0; Q9UJ58; Q9UJ59; Q9UJ60; B2RMX8
• Molecular Weight: 37,976 Da
• NCBI Official Full Name: apoptotic protease-activating factor 1 isoform b
• NCBI Official Synonym Full Names: apoptotic peptidase activating factor 1
• NCBI Official Symbol: APAF1
• NCBI Official Synonym Symbols: CED4; APAF-1
• NCBI Protein Information: apoptotic protease-activating factor 1
• UniProt Protein Name: Apoptotic protease-activating factor 1
• UniProt Gene Name: APAF1
• UniProt Synonym Gene Names: KIAA0413; APAF-1

NCBI Description

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.