CNTF recombinant protein

Human CNTF protein

Cat. Num. AAA9718991

• Gene Names: CNTF; HCNTF
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CNTF; Human CNTF protein; Ciliary Neurotrophic Factor; Ciliary Neuronotrophic Factor; HCNTF; CNTF recombinant protein

• Host: E Coli
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5.
• Sequence: Ala2-Met200

• Endotoxin: <0.1 EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from Human CNTF(P26441)(Ala2-Met200) was expressed.
• Protein Length: The recombinant Human CNTF consists of 199 amino acids and has a predicted molecular mass of 22kDa.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CNTF protein using the buffer we provided not less than 100ug/mL, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Human CNTF protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human CNTF protein.)

Related Product Information for CNTF recombinant protein

Description: Recombinant Human CNTF protein expressed in E Coli.
Background: Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. CNTF is a survival factor for various neuronal cell types. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons.

Product Categories/Family for CNTF recombinant protein

Cell Culture; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4758020
• NCBI GeneID: 1270
• NCBI Accession #: NP_000605.1
• NCBI GenBank Nucleotide #: NM_000614.3
• UniProt Accession #: P26441; B2RAB2
• Molecular Weight: 22,931 Da
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: CNTF
• NCBI Official Synonym Symbols: HCNTF
• NCBI Protein Information: ciliary neurotrophic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: CNTF
• UniProt Synonym Gene Names: CNTF
• UniProt Entry Name: CNTF_HUMAN

NCBI Description

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

Uniprot Description

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: interleukin-6 receptor binding; growth factor activity; ciliary neurotrophic factor receptor binding

Biological Process: negative regulation of photoreceptor cell differentiation; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; signal transduction; muscle morphogenesis; growth; positive regulation of tyrosine phosphorylation of Stat3 protein