growth hormone Active Protein | chGH active protein
Chicken growth hormone
Gene Names
GH1; GH
Purity
The purity of chicken recombinant growth hormone is greater than 99.0% as determined by:(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
Synonyms
growth hormone; Chicken growth hormone; Chicken growth hormone (chGH); chGH active protein
Host
E Coli
Purity/Purification
The purity of chicken recombinant growth hormone is greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids of chicken recombinant growth hormone was determined and was found to be Ala-Thr-Phe-Pro-Ala.
Sequence Length
216
Solubility
It is recommended to reconstitute the lyophilized chicken recombinant growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Protein Content
Protein quantitation of chicken recombinant growth hormone was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the DNAman program.
Dimers and Aggregates
The purified chicken recombinant growth hormone (22K) consists of > 99% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of chicken recombinant growth hormone.
Formulation
The chicken recombinant growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.
Biological Activity
Chicken recombinant growth hormone is fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit growth hormonr receptors.
Preparation and Storage
Lyophilized chicken recombinant growth hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution of chicken recombinant growth hormone at > 0.1 mg/ml and up to 3 mg/ml and filter sterilization chicken recombinant growth hormone can be stored at 4 degree C for several weeks. At lower concentration of chicken recombinant growth hormone addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles.
Related Product Information for chGH active protein
Chicken recombinant growth hormone (chGH) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22237 Dalton. Chicken recombinant growth hormone is purified by proprietary chromatographic techniques. For reference see Eliasiewicz et al. (2006) Ann N Y Acad Sci. 1091:501-508.
Product Categories/Family for chGH active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,423 Da
NCBI Official Full Name
somatotropin
NCBI Official Symbol
GH1
NCBI Official Synonym Symbols
GH
NCBI Protein Information
somatotropin
UniProt Protein Name
Somatotropin
Protein Family
UniProt Gene Name
GH1
UniProt Entry Name
SOMA_HORSE
Uniprot Description
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Research Articles on chGH
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Product Notes
The chGH gh1 (Catalog #AAA400012) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids of chicken recombinan t growth hormone was determined and was found to be Ala-Thr-Ph e-Pro-Ala. It is sometimes possible for the material contained within the vial of "growth hormone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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