Human Soluble CD36 ELISA Kit | sCD36 elisa kit
Human Soluble CD36 ELISA Kit
Reactivity
Human
Synonyms
Soluble CD36; Human Soluble CD36 ELISA Kit; sCD36 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of sCD36. No significant cross-reactivity or interference between sCD36 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sCD36 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
()
Related Product Information for sCD36 elisa kit
Intended Uses: This sCD36 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumansCD36. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: sCD36 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sCD36 antibody and an sCD36-HRP conjugate. The assay sample and buffer are incubated together with sCD36-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD36 concentration since sCD36 from samples and sCD36-HRP conjugate compete for the anti-sCD36 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD36 from the sample, fewer sites are left to bind sCD36-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD36 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: sCD36 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sCD36 antibody and an sCD36-HRP conjugate. The assay sample and buffer are incubated together with sCD36-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD36 concentration since sCD36 from samples and sCD36-HRP conjugate compete for the anti-sCD36 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD36 from the sample, fewer sites are left to bind sCD36-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD36 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for sCD36 elisa kit
