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Immunohistochemistry (IHC) (Immunochemical staining of human CA5B in human placenta with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Rabbit anti-Human Carbonic Anhydrase VB Monoclonal Antibody | anti-CA5B antibody

Recombinant Anti-Carbonic Anhydrase VB Antibody, Rabbit Monoclonal

Gene Names
CA5B; CA-VB
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A
Synonyms
Carbonic Anhydrase VB; Monoclonal Antibody; Recombinant Anti-Carbonic Anhydrase VB Antibody; Rabbit Monoclonal; Carbonic Anhydrase VB/CA5B Antibody; Rabbit MAb; carbonic anhydrase VB; mitochondrial; Anti-CA-VB Antibody; Anti-MGC39962 Antibody; anti-CA5B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
008
Specificity
Human Carbonic Anhydrase VB/CA5B.
No cross-reactivity in ELISA with Human CA8, Human CA4
Has cross-reactivity in ELISA with Human CA2, Human CA3, Human CA5A
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CA5B antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human Carbonic Anhydrase VB/CA5B protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase VB/CA5B (rh Carbonic Anhydrase VB/CA5B).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CA5B in human placenta with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human CA5B in human placenta with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CA5B in human lung with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human CA5B in human lung with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CA5B in human kidney with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human CA5B in human kidney with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-CA5B antibody
Carbonic anhydrase 5B, also known as carbonate dehydratase VB, carbonic anhydrase VB, CA-VB and CA5B, is amember of the alpha-carbonic anhydrase family. The strongest expression of CA5B/CA-VB is in heart, pancreas, kidney, placenta, lung, and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B/CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A/CA-VA. CA5B/CA-VB has a wider tissue distribution than CA5A/CA-VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A/CA-VA is activated by histamine, L-adrenaline, L-and D-histidine, and L-and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B/CA-VB is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin and Foscarnet (phosphonoformate trisodium salt).
References
Fujikawa-Adachi K, et al., 1999, J Biol Chem. 274 (30): 21228-33. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257-262. Temperini C.et al., 2006, Chemistry 12: 7057-66. Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222. Maresca, A.et al., 2009, J. Am. Chem. Soc. 131:3057-3062.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,434 Da
NCBI Official Full Name
carbonic anhydrase 5B, mitochondrial
NCBI Official Synonym Full Names
carbonic anhydrase VB, mitochondrial
NCBI Official Symbol
CA5B
NCBI Official Synonym Symbols
CA-VB
NCBI Protein Information
carbonic anhydrase 5B, mitochondrial; carbonic dehydratase; carbonate dehydratase VB
UniProt Protein Name
Carbonic anhydrase 5B, mitochondrial
Protein Family
UniProt Gene Name
CA5B
UniProt Synonym Gene Names
CA-VB
UniProt Entry Name
CAH5B_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

CA5B: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1; Mitochondrial

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process

Research Articles on CA5B

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Product Notes

The CA5B ca5b (Catalog #AAA8106361) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Carbonic Anhydrase VB Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase VB can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the CA5B ca5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase VB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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