Vimentin (VIM) Active Protein | VIM active protein

Active Vimentin (VIM)

Cat. Num. AAA2125335

• Applications: Cell culture, Activity Assays
• Purity: Greater than 97% by SDS-PAGE
• Synonyms: Vimentin (VIM); Active Vimentin (VIM); VIM/Vimentin; VIM active protein

• Host: E Coli
• Purity/Purification: Greater than 97% by SDS-PAGE
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Ser2~Glu466

• Applicable Applications for VIM active protein: Cell culture, Activity Assays
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Tag: N-terminal His Tag
• Biological Activity: Active
• Expression System: Prokaryotic expression
• Organism Species: Human
• Isoelectric Point: 5.2
• Subcellular Location: Secreted
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

Gene Sequencing

(Gene Sequencing (extract))

SDS-Page

(SDS-PAGE)

Activity

(Vimentin (VIM) is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Besides, Keratin 20 (KRT20) has been identified as an interactor of VIM, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VIM and recombinant human KRT20. Briefly, VIM were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100?l then transferred to KRT20-coated microtiter wells and incubated for 2h at 37?. Wells were washed with PBST and incubated for 1h with anti-VIM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37?. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of VIM and KRT20 was shown in Figure 1, and this effect was in a dose dependent manner.)

Sequence

Related Product Information for VIM active protein

Vimentin (VIM) is a type III intermediate filament (IF) protein that is expressed inmesenchymal cells. Vimentin is the major cytoskeletal component ofmesenchymal cells. Because of this, vimentin is often used as a marker ofmesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymaltransition (EMT) during both normal development and metastatic progression. Besides, Keratin 20 (KRT20) has been identified as an interactor of VIM, thus abinding ELISA assay was conducted to detect the interaction of recombinanthuman VIM and recombinant human KRT20. Briefly, VIM were diluted serially inPBS, with 0.01% BSA (pH7.4). Duplicate samples of 100ul then transferred to KRT20-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washedwith PBST and incubated for 1h with anti-VIM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells wereaspirated and washed 3 times. With the addition of substrate solution, wells wereincubated 15-25 minutes at 37 degree C. Finally, add 50ul stop solution to the wells andread at 450nm immediately. The binding activity of VIM and KRT20 was shown in Figure 1, and this effect was in a dose dependent manner.

Product Categories/Family for VIM active protein

Tumor immunity; Developmental science; Active, prokaryotic recombinant

NCBI and Uniprot Product Information

• NCBI GI #: 62414289
• NCBI GeneID: 7431
• NCBI Accession #: NP_003371.2
• NCBI GenBank Nucleotide #: NM_003380.3
• UniProt Accession #: P08670; Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
• Molecular Weight: 53,652 Da
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Protein Information: vimentin
• UniProt Protein Name: Vimentin
• Protein Family: Vimentin
• UniProt Gene Name: VIM
• UniProt Entry Name: VIME_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Uniprot Description

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30