Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Regenerating gene 4 (REG-4) Standard Curve.)
Human Regenerating Gene 4 (REG-4) ELISA Kit | REG-4 elisa kit
Human Regenerating Gene 4 (REG-4) ELISA Kit
Gene Names
REG4; GISP; RELP; REG-IV
Reactivity
Human
Synonyms
Regenerating Gene 4 (REG-4); Human Regenerating Gene 4 (REG-4) ELISA Kit; REG4; GISP; REG-IV; RELP; OTTHUMP00000014031; gastrointestinal secretory protein; regenerating gene type IV; REG-4 elisa kit
Reactivity
Human
Specificity
Human Regenerating gene 4 (REG-4) ELISA Kit has high sensitivity and excellent specificity for detection of Human REG4. No significant cross-reactivity or interference between Human REG4 and analogues was observed.
Sequence Length
134
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Regenerating gene 4 (REG-4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Regenerating gene 4 (REG-4) Standard Curve.)
Related Product Information for REG-4 elisa kit
Description: This Human Regenerating gene 4 (REG-4) ELISA Kit employs a two-site sandwich ELISA to quantitate REG4 in samples. An antibody specific for REG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The REG4 gene encodes a deduced 158-amino acid protein with an N-terminal signal peptide of 22 amino acids and a predicted molecular mass of 18.2 kD. REG4 maintains conservation of the 6 cysteine residues present in other known members of the REG family. By Northern blot analysis of intestinal mucosa from 12 individuals with inflammatory bowel disease, Hartupee et al. (2001) identified a 1.1-kb REG4 transcript in all samples. Control specimens had low but detectable expression of REG4. REG4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis. RNA dot-blot analysis showed that most of the tissues expressing REG4 are in the gastrointestinal tract, i.e., colon, small intestine, stomach, and pancreas.
Background: The REG4 gene encodes a deduced 158-amino acid protein with an N-terminal signal peptide of 22 amino acids and a predicted molecular mass of 18.2 kD. REG4 maintains conservation of the 6 cysteine residues present in other known members of the REG family. By Northern blot analysis of intestinal mucosa from 12 individuals with inflammatory bowel disease, Hartupee et al. (2001) identified a 1.1-kb REG4 transcript in all samples. Control specimens had low but detectable expression of REG4. REG4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis. RNA dot-blot analysis showed that most of the tissues expressing REG4 are in the gastrointestinal tract, i.e., colon, small intestine, stomach, and pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
regenerating islet-derived protein 4 isoform 2
NCBI Official Synonym Full Names
regenerating family member 4
NCBI Official Symbol
REG4
NCBI Official Synonym Symbols
GISP; RELP; REG-IV
NCBI Protein Information
regenerating islet-derived protein 4
UniProt Protein Name
Regenerating islet-derived protein 4
UniProt Gene Name
REG4
UniProt Synonym Gene Names
GISP; RELP; REG-4; Reg IV
UniProt Entry Name
REG4_HUMAN
Uniprot Description
REG4: Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p13.1-p12
Cellular Component: cytoplasm; extracellular region
Molecular Function: heparin binding; calcium ion binding
Research Articles on REG-4
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Product Notes
The Human REG-4 reg4 (Catalog #AAA9714477) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714477 ELISA Kit recognizes Human REG-4. It is sometimes possible for the material contained within the vial of "Regenerating Gene 4 (REG-4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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