UBC9 active protein
Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9)
Purity
Highly Purified95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
Ubc9; Recombinant; Human (Ubiquitin Conjugating Enzyme 9); UBC9 active protein
Host
E Coli
Purity/Purification
Highly Purified
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 100mM sodium chloride, 100mM imidazole, 0.5mM PMSF, 1mM DTT, 10% glycerol.
Biological Activity
IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10e6 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of ubiquitin-conjugating enzyme
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm
2. Analysis by RP-HPLC, using a standard solution of UBC-9 as a Reference Standard.
1. UV spectroscopy at 280nm
2. Analysis by RP-HPLC, using a standard solution of UBC-9 as a Reference Standard.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for UBC9 active protein
Human UBC9 Ubiquitin-like protein SUMO-1 conjugating enzyme is also called UBE2I. rHuUBC9 Mediates the conjugation of the ubiquitin-like protein SUMO-1 to a variety of proteins including RanGAP1, IkappaB alpha, and PML without the requirement of an ubiquitin ligase (E3).
Recombinant Human ubiquitin-conjugating enzyme is a single, non-glycosylated, polypeptide chain having a molecular mass of 23kD.
Recombinant Human ubiquitin-conjugating enzyme is a single, non-glycosylated, polypeptide chain having a molecular mass of 23kD.
Product Categories/Family for UBC9 active protein
NCBI and Uniprot Product Information
Uniprot Description
Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5
Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.
Pathway: Protein modification; protein sumoylation.
Subunit structure: Interacts with SIZ1. Ref.5 Ref.6
Subcellular location: Nucleus Ref.7.
Miscellaneous: Present with 2600 molecules/cell in log phase SD medium.
Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
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Product Notes
The Ubc9 ubc9 (Catalog #AAA635888) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ubc9, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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