Tumor Necrosis Factor Receptor I Active Protein | TNFRSF1A active protein

Recombinant Human Tumor Necrosis Factor Receptor I Protein

Cat. Num. AAA2556695

• Gene Names: TNFRSF1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Tumor Necrosis Factor Receptor I; Recombinant Human Tumor Necrosis Factor Receptor I Protein; Tumor Necrosis Factor Receptor Superfamily Member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; TNFAR; TNFR1; TNFRSF1A active protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 455

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Ile22-Thr211
• Fusion Tag: N-6His
• Activity: Measured by its binding ability in a functional ELISA.Immobilized human TNFRI-His at 10 mug/mL (100 mul/well) can bind biotinylated human TNFalpha. The EC50 of biotinylated human TNFalpha is 5-12 mug/mL. 2. Measured by its ability to inhibit TNFalpha-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1mug/mL in the presence of 0.25 ng/mL recombinant human TNFalpha.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-Page

Related Product Information for TNFRSF1A active protein

Protein Construction: Recombinant Human Tumor Necrosis Factor Receptor I is produced by our E Coli expression system and the target gene encoding Ile22-Thr211 is expressed with a 6His tag at the N-terminus.

Background: Tumor necrosis factor receptor superfamily member 1A (Tnfrsf1a) is a member of the tumor necrosis factor receptor superfamily. Tnfrsf1a is one of the major receptors for the tumor necrosis factor-alpha. It can activate the transcription factor NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the human genetic disorder called tumor necrosis factor associated periodic syndrome (TRAPS) or periodic fever syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 4507575
• NCBI GeneID: 7132
• NCBI Accession #: NP_001056.1
• NCBI GenBank Nucleotide #: NM_001065.3
• UniProt Accession #: P19438; Q9UCA4; A8K4X3; B2RDE4; B3KPQ1; B4DQB7; B4E309; B5M0B5; D3DUR1
• Molecular Weight: Molecular Mass: 23.5kDa; Actual Protein Molecular Mass: 22kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1A isoform 1
• NCBI Official Synonym Full Names: TNF receptor superfamily member 1A
• NCBI Official Symbol: TNFRSF1A
• NCBI Official Synonym Symbols: FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF1A
• UniProt Synonym Gene Names: TNFAR; TNFR1; TNF-R1; TNF-RI; TNFR-I; TBPI

NCBI Description

This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016]

Uniprot Description

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.