Rabbit anti-Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide Polyclonal Antibody | anti-ATP1a1 antibody

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody

Cat. Num. AAA2032208

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: ATPase; Na+/K+ Transporting Alpha 1 Polypeptide; Polyclonal Antibody; Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody; Sodium pump subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium/potassium-transporting ATPase subunit alpha-1; anti-ATP1a1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against ATP1a1. It has been selected for its ability to recognize ATP1a1 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 1023

• Applicable Applications for anti-ATP1a1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western Blot: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunoglobulin Type: IgG
• Conjugate: No Conjugate
• Cross Reactivity: Human
• Organism Species: Human
• Fragment: ATP1a1 (His493~Ala660)
• Quality Control: Content: The quality control contains recombinant ATP1a1 (His493~Ala660) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2077942
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant ATP1a1, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Human Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Human Glioma Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z2T5; B7Z3U6; F5H3A1
• Molecular Weight: 113,000 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit alpha 1
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: EC 3.6.3.9; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; endoplasmic reticulum; endosome; extracellular exosome; Golgi apparatus; integral component of membrane; intercalated disc; melanosome; membrane; myelin sheath; plasma membrane; postsynaptic density; protein complex; sarcolemma; sodium:potassium-exchanging ATPase complex; T-tubule

Molecular Function: ADP binding; ankyrin binding; ATP binding; chaperone binding; phosphoinositide 3-kinase binding; phosphoric monoester hydrolase activity; potassium ion binding; protein binding; protein domain specific binding; protein kinase binding; sodium ion binding; sodium:potassium-exchanging ATPase activity; steroid hormone binding

Biological Process: cardiac muscle cell action potential involved in contraction; cell communication by electrical coupling involved in cardiac conduction; cellular potassium ion homeostasis; cellular response to steroid hormone stimulus; cellular sodium ion homeostasis; dephosphorylation; establishment or maintenance of transmembrane electrochemical gradient; membrane hyperpolarization; membrane repolarization; membrane repolarization during cardiac muscle cell action potential; negative regulation of glucocorticoid biosynthetic process; negative regulation of heart contraction; positive regulation of heart contraction; positive regulation of striated muscle contraction; potassium ion import; potassium ion import across plasma membrane; regulation of blood pressure; regulation of cardiac muscle cell contraction; regulation of sodium ion transport; regulation of the force of heart contraction; response to drug