TNFSF15 active protein

Recombinant Human TNFSF15

Cat. Num. AAA4159843

• Gene Names: TNFSF15; TL1; TL1A; VEGI; TNLG1B; VEGI192A
• Synonyms: TNFSF15; Recombinant Human TNFSF15; TL1; VEGI; TNFSF15 active protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 micron filtered solution in Tris and salt
• Sequence Length: 251

• Bioactivity: The ED(50) was determined by the dose-dependent proliferation of Jurkat cells and was found to be <0.5ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL is recommended. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for TNFSF15 active protein

TNFSF15 is a type II transmembrane protein which is predominantly expressed in endothelial cells. It has a single transmembrane domain, a small cytoplasmic domain and an extracellular C-terminus. Its expression is stimulated by TNFA and IL1A. TNFSF15 acts as the ligand for the receptor protein TNFRSF25 which is primarily found in activated T cells. It also has an affinity to the decoy receptor protein TNFRSF6B. Binding of this cytokine to its receptor can activate the transcription factor NF-Kappa-B and MAP Kinases. It has been found that TNFSF15 can also induce apoptosis by acting as an autocrine factor and inhibit endothelial cell proliferation. Recombinant Human TNFSF15 herein refers to the secreted form of the protein.

NCBI and Uniprot Product Information

• NCBI GI #: 23510445
• NCBI GeneID: 9966
• NCBI GenBank Nucleotide #: NP_005109.2
• UniProt Accession #: O95150
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 15 isoform VEGI-251
• NCBI Official Synonym Full Names: TNF superfamily member 15
• NCBI Official Symbol: TNFSF15
• NCBI Official Synonym Symbols: TL1; TL1A; VEGI; TNLG1B; VEGI192A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 15
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 15
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF15
• UniProt Synonym Gene Names: TL1; VEGI
• UniProt Entry Name: TNF15_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFSF15: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; cytokine metabolic process; activation of NF-kappaB-inducing kinase; immune response; signal transduction; positive regulation of cytokine secretion