TNFSF12 active protein

TNFSF12

Cat. Num. AAA553631

• Gene Names: TNFSF12; APO3L; DR3LG; TWEAK; TNLG4A
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: TNFSF12; TNFSF12 active protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH
• Sequence Length: 249

• Applicable Applications for TNFSF12 active protein: SDS-PAGE
• Application Notes: Recombinant TNFSF12 migrates as an approximately 17 kDa protein under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human TNFSF12 mature chain was expressed in ECOLI. Recombinant Human Tumor necrosis factor ligand superfamily member 12 encodes amino acid residues 1-249.
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 4507597
• NCBI GeneID: 8742
• NCBI Accession #: NP_003800.1
• NCBI GenBank Nucleotide #: NM_003809.3
• UniProt Accession #: O43508; Q8IZK7; Q8WUZ7
• Molecular Weight: 36,589 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 12 proprotein
• NCBI Official Synonym Full Names: TNF superfamily member 12
• NCBI Official Symbol: TNFSF12
• NCBI Official Synonym Symbols: APO3L; DR3LG; TWEAK; TNLG4A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 12
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 12
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF12
• UniProt Synonym Gene Names: APO3L; DR3LG; TWEAK

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.