Rabbit Adrenergic Receptor, Alpha 1A ELISA Kit | ADRalpha1A elisa kit

Rabbit Adrenergic Receptor, Alpha 1A ELISA Kit

Cat. Num. AAA720483

• Gene Names: ADRA1D; DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
• Reactivity: Rabbit
• Synonyms: Adrenergic Receptor; Alpha 1A; Rabbit Adrenergic Receptor; Alpha 1A ELISA Kit; ADRalpha1A elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ADR?1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit ADR?1A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADRalpha1A elisa kit

Principle of the assay: ADR?1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADR?1A antibody and an ADR?1A-HRP conjugate. The assay sample and buffer are incubated together with ADR?1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADR?1A concentration since ADR?1A from samples and ADR?1A-HRP conjugate compete for the anti-ADR?1A antibody binding site. Since the number of sites is limited, as more sites are occupied by ADR?1A from the sample, fewer sites are left to bind ADR?1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADR?1A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADRalpha1A elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 7690135
• NCBI GeneID: 146
• Molecular Weight: 60,463 Da
• NCBI Official Full Name: alpha adrenergic receptor subtype alpha 1a
• NCBI Official Synonym Full Names: adrenoceptor alpha 1D
• NCBI Official Symbol: ADRA1D
• NCBI Official Synonym Symbols: DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
• NCBI Protein Information: alpha-1D adrenergic receptor; alpha-1D adrenoceptor; alpha-1D adrenoreceptor; alpha-1A adrenergic receptor; alpha-1D-adrenergic receptor; alpha-adrenergic receptor 1a; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1D-, receptor; adrenergic, alpha -1D-, receptor
• UniProt Protein Name: Alpha-1D adrenergic receptor
• UniProt Gene Name: ADRA1D
• UniProt Synonym Gene Names: ADRA1A; Alpha-1D adrenoceptor
• UniProt Entry Name: ADA1D_HUMAN

NCBI Description

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1D-adrenergic receptor. Similar to alpha-1B-adrenergic receptor gene, this gene comprises 2 exons and a single intron that interrupts the coding region. [provided by RefSeq, Jul 2008]

Uniprot Description

ADRA1D: This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1D sub-subfamily.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: alpha1-adrenergic receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; multicellular organismal development; positive regulation of cell proliferation; negative regulation of the force of heart contraction during baroreceptor response to increased systemic arterial blood pressure; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; DNA metabolic process