Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Tumor Necrosis Factor Receptor Superfamily Polyclonal Antibody | anti-TNFRSF25 antibody
Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25)
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Tumor Necrosis Factor Receptor Superfamily; Polyclonal Antibody; Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily; Member 25 (TNFRSF25); TRAMP; TR3; APO-3; DDR3; DR3; LARD; TNFRSF12; WSL-1; WSL-LR; Translocating Chain-Association Membrane Protein; Lymphocyte-associated receptor of death; anti-TNFRSF25 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TNFRSF25. It has been selected for its ability to recognize TNFRSF25 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-TNFRSF25 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
TNFRSF25 (Cys38~Gly167)
Conjugate
No Conjugate
Cross Reactivity
Rat
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant TNFRSF25 (Cys38~Gly167) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073561
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded intestine tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded intestine tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,090 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 25
NCBI Official Synonym Full Names
TNF receptor superfamily member 25
NCBI Official Symbol
Tnfrsf25
NCBI Protein Information
tumor necrosis factor receptor superfamily member 25
UniProt Protein Name
Similar to tumor necrosis factor receptor superfamily, member 25, isoform CRA_a
Protein Family
UniProt Gene Name
Tnfrsf25
Uniprot Description
TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 5q36
Cellular Component: integral component of plasma membrane; plasma membrane
Molecular Function: tumor necrosis factor-activated receptor activity
Biological Process: immune response; inflammatory response; multicellular organism development; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway
Similar Products
Product Notes
The TNFRSF25 tnfrsf25 (Catalog #AAA2034077) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Receptor Superfamily can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the TNFRSF25 tnfrsf25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Receptor Superfamily, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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