Sulfotransferase 2A1 (Sult2a1) Active Protein | Sult2a1 active protein
Sulfotransferase 2A1, Recombinant, Human (SULT2A1, Bile salt sulfotransferase, Hydroxysteroid Sulfotransferase, HST, Dehydroepiandrosterone sulfotransferase, DHEA-ST, ST2, ST2A3, STD)
Applications
Western Blot
Purity
95% by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
Sulfotransferase 2A1 (Sult2a1); Sulfotransferase 2A1; Recombinant; Human (SULT2A1; Bile salt sulfotransferase; Hydroxysteroid Sulfotransferase; HST; Dehydroepiandrosterone sulfotransferase; DHEA-ST; ST2; ST2A3; STD); Sult2a1 active protein
Host
E. coli
Purity/Purification
95% by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a liquid in 25mM Tris, pH 7.5, 250mM sodium chloride, 1mM DTT.
Concentration
0.231 mg/ml (varies by lot)
Applicable Applications for Sult2a1 active protein
Western Blot (WB) - Other applications not tested.
Application Notes
Optimal dilutions to be determined by the researcher.
Host Note
Recombinant enzyme corresponding to Ser2-Glu285 with a Met and N-terminal 6 His tag of human Sulfotransferase 2A1 expressed in E. coli.
Endotoxin
<1.0EU/ug (LAL)
Activity
Measured by its ability to transfer sulfate from PAPS to tDehydroandrosterone (DHEA).
Specific Activity
>15pmol/min/ug
N-Terminal Sequence Analysis:
Met
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Sult2a1 active protein
Cytosolic sulfotransferases are a family of Phase II drugmetabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (13). They have important roles in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase watersolubility of xenobiotics and therapeutic drugs. They are distinct from Golgi resident sulfotransferases by lacking Nterminal signalanchor domains and residing only in the cytoplasm. SULT2A1 is primarily expressed in liver and adrenal tissues where it sulfates steroids and bile acids (46). It is also called DHEA/bile acid sulfotransferase and plays roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and, at the same time, preventing toxicity from bile acid accumulation.
Product Categories/Family for Sult2a1 active protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~36kD; Predicted Molecular Mass: 35kD; SDS-PAGE: 36kD, reducing conditions
Protein Family
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Product Notes
The Sult2a1 (Catalog #AAA637726) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Sulfotransferase 2A1 (Sult2a1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) - Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Sult2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sulfotransferase 2A1 (Sult2a1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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